Bacillus salidurans sp. nov., isolated from salt-accumulated pepper rhizospheric soil. Issue 1 (14th November 2018)

• Record Type: Journal Article
• Authors: Son, Jin-Soo; Hwang, Ye-Ji; Lee, Soo-Yeong; Ghim, Sa-Youl
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 69:Issue 1(2019)
• Page Start: 116
• Page End: 122
• Publication Date: 2018-11-14
• Subjects: Bacillus -- pepper rhizosphere; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.003110
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : A Gram-stain-positive, facultatively anaerobic, motile and rod-shaped bacterium, designated KNUC7312 T, was isolated from salt-accumulated rhizospheric soil in a pepper greenhouse in Miryang city, Republic of Korea. Cell growth of strain KNUC7312 T occurred at 10–45 °C (optimum, 30 °C) and pH 7–12 (optimum, pH 7). In addition, this strain was able to tolerate 0–12 % NaCl (w/v) concentration (optimum, 0–1 %). Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain KNUC7312 T clustered together with other species of the genus Bacillus and was most closely related to Bacillus humi DSM 16318 T (98.0 %). The predominant respiratory quinone was menaquinone-7 (MK-7). The major cellular fatty acids were anteiso C15 : 0, iso-C15 : 0 and iso-C14 : 0 . The polar lipid profile contained the major components diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and two unidentified aminolipids. The cell-wall peptidoglycan contained meso -diaminopimelic acid as the major diagnostic diamino acid. Strain KNUC7312 T showed a low DNA–DNA relatedness value (47.36 %) with B. humi DSM 16318 T, which supported that this strain represents a novel Bacillus species. On the basis of phenotypic, chemotaxonomic and phylogenetic evidence, strain KNUC7312 T represents a novel species within the genera Bacillus . The name Bacillus salildurans sp. nov. is proposed. The type strain is KNUC7312 T (KCTC 33852 T =CGMCC 1.13629 T ).