An assessment workflow to recover microplastics from complex biological matrices. (June 2022)

• Record Type: Journal Article
• Authors: F.M. Santana, Marina; Kroon, Frederieke J.; van Herwerden, Lynne; Vamvounis, George; Motti, Cherie A.
• Is Part Of: Marine pollution bulletin. Volume 179(2022)
• Publication Date: 2022-06
• Subjects: Marine environment -- Clarification efficiency -- Microplastic integrity -- Recovery rates -- Invertebrates; Marine pollution -- Periodicals; Marine Biology -- Periodicals; Water Pollution -- Periodicals; Mer -- Pollution -- Périodiques; Publications périodiques; Pollution des mers; Lutte antipollution; Electronic journals; 363.7394
• DOI: 10.1016/j.marpolbul.2022.113676
• Languages: English
• ISSNs: 0025-326X

Abstract

Abstract: A criteria-guided workflow was applied to assess the effectiveness of microplastic separation methods on complex marine biological matrices. Efficacy of four methods (nitric acid, HNO3, and potassium hydroxide, KOH, digestions, and sodium chloride, NaCl, and potassium iodide, KI, density flotations) was evaluated on four taxa (hard coral, sponge, sea squirt, sea cucumber) using five microplastics (polyethylene, polystyrene, polyethylene terephthalate, PET, polyvinylchloride, rayon). Matrix clarification was only unacceptably low for KOH. PET discoloured regardless of reagent. Rayon threads unravelled into monofilaments after exposure to all reagents, with discolouration also occurring with HNO3 . Recovery rates were overall high, except for dense microplastics treated with NaCl and only KI yielded high rayon recovery efficiency. All polymers were accurately assigned, with subtle spectral changes observed. These results demonstrate specific limitations to separation methods applied to different biological matrices and microplastics and highlight the need to assess their suitability to provide estimates of microplastic contamination. Graphical abstract: Unlabelled Image Highlights: Methods of microplastic separation were assessed for four underreported marine taxa. The applied workflow identified specific limitations for each separation method. Identified limitations were taxon and/or microplastic specific.