Assembly of redox active metallo-enzymes and metallo-peptides on electrodes: Abiological constructs to probe natural processes. (June 2022)

• Record Type: Journal Article
• Authors: Sarkar, Ankita; Chattopadhyay, Samir; Mukherjee, Manjistha; Ghosh Dey, Somdatta; Dey, Abhishek
• Is Part Of: Current opinion in chemical biology. Volume 68(2022)
• Publication Date: 2022-06
• Subjects: Abiological construct -- Bio-inspired electrodes -- Self-assembled monolayers -- Cytochrome c -- Cytochrome c oxidase -- Aβ peptide -- Electron transfer -- O2 reduction -- Aβ–mediated toxicity -- Partially reduced oxygen species (PROS) -- Proton transfer residues -- Solvent kinetic isotope effect; Bioorganic chemistry -- Periodicals; Biology -- Periodicals; Biochemistry -- Periodicals; Clinical biochemistry -- Periodicals; Biochemistry -- Periodicals; Chimie bio-organique -- Périodiques; Biologie -- Périodiques; 572.05
• DOI: 10.1016/j.cbpa.2022.102142
• Languages: English
• ISSNs: 1367-5931

Abstract

Abstract: Redox active metallo-proteins and metallo-peptides attached to self-assembled monolayers (SAM) of thiols on Au electrodes or constituting the SAM on Au electrodes can provide unique opportunities to investigate a range of complicated biological phenomena in controlled abiological constructs. In addition to conventional biochemical tools like site-directed mutagenesis, these constructs allow control over electron transfer (ET) processes, micro solvation (SAM design), folding/misfolding and orientation of these biological entities. This article presents a review of the work done by this group in creating abiological bio-inspired SAM on Au electrodes to probe several important biological processes where redox plays or might play a major role. These include stabilisation of different morphologies of Aβ peptides and which allow investigation of the reactivity of their Cu/Zn/heme-bound forms, determination of both outer-sphere and inner-sphere reorganisation energies of cytochrome c along with deciphering the role of the fluxional methionine and finally creation of bio-chemical constructs of cytochrome c oxidase which not only reduce O2 selectively to H2 O efficiently but also provide key insights in O2 reduction mechanism which has aided the development of efficient artificial catalysts. Graphical abstract: Image 1