Designed protease-based signaling networks. (June 2022)

• Record Type: Journal Article
• Authors: Fink, Tina; Jerala, Roman
• Is Part Of: Current opinion in chemical biology. Volume 68(2022)
• Publication Date: 2022-06
• Subjects: Proteolysis -- Viral proteases -- Endogenous proteases -- Protease-based sensors -- Synthetic signaling cascades; Bioorganic chemistry -- Periodicals; Biology -- Periodicals; Biochemistry -- Periodicals; Clinical biochemistry -- Periodicals; Biochemistry -- Periodicals; Chimie bio-organique -- Périodiques; Biologie -- Périodiques; 572.05
• DOI: 10.1016/j.cbpa.2022.102146
• Languages: English
• ISSNs: 1367-5931

Abstract

Abstract: Regulated proteolysis is a pivotal regulatory mechanism in all living organisms from bacteria to mammalian cells and viruses. The ability to design proteases to sense, transmit, or trigger a signal opens up the possibility of construction of sophisticated proteolysis-regulated signaling networks. Cleavage of the polypeptide chain can either activate or inactivate the selected protein or process, often with a fast response. Most designs are based on sequence-selective proteases that can be implemented for transcriptional, translational, and ultimately post-translational control, aiming to engineer complex circuits that can dynamically control cellular functions and enable novel biotechnological and biomedical applications.