LED 209 conjugated chitosan as a selective antimicrobial and potential anti-adhesion material. (15th February 2019)

• Record Type: Journal Article
• Authors: Zhou, Zongbao; Chen, Tao; Mei, Naibin; Li, Bing; Xu, Zunkai; Wang, Lei; Wang, Xiaoying; Tang, Shunqing
• Is Part Of: Carbohydrate polymers. Volume 206(2019)
• Page Start: 653
• Page End: 663
• Publication Date: 2019-02-15
• Subjects: Chitosan -- LED 209 -- Multidrug-resistant gram-negative bacteria -- Selective activity -- Anti-adhesion activity; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2018.11.044
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: LED 209 conjugated CS as the novel selective antimicrobial and anti-adhesion agent. The conjugate exhibited higher selective antimicrobial on MDR- E. coli than CS. The conjugate could reduce MDR- E. coli adhesion and prevent its biofilm formation. The conjugate offered a potent antimicrobial for combating GNB infections. Graphical abstract: Abstract: The rapid emergence of antibiotic-resistant Gram-negative bacteria (GNB) is becoming a global healthcare concern, and it urgently needs novel strategies to match the clinical challenge. In this work, we conjugated chitosan (CS) with LED 209, a highly selective inhibitor of QseC of GNB, to create the novel selective antimicrobial agent CS/LED. The data of FT-IR, NMR and elemental analysis for CS/LED conjugates proved the successful conjugation of CS with LED 209. Interestingly, the fluorescence signal detected in MDR- E. coli of CS/LED-FITC was about 2 times than that of CS-FITC at 3 h. The results shown that compared with CS, CS/LED exhibited higher selective antimicrobial on MDR- E. coli . Moreover, CS/LED exhibited the lower selectivity and cytotoxicity to mammalian cell than CS. Additionally, an unexpected enhancement of anti-adhesion activity against MDR- E. coli was determined by cellulose membrane coating CS/LED. The results demonstrated that CS/LED could reduce the adhesion of bacteria to the cellulose membrane by about 67.8%, while CS only reduced by about 45.3%. The dressings coated with CS/LED possessed the stronger ability to prevent microbial adhesion compared to the CS-coated dressing. Our present work firstly demonstrated that CS/LED had a highly selective activity and anti-adhesion activity against MDR- E. coli, which offered a potent and selective antimicrobial for combating multidrug-resistant GNB infections.