Plasma proteomics reveals an improved cardio-metabolic profile in patients with type 2 diabetes post-liraglutide treatment. (May 2022)

• Record Type: Journal Article
• Authors: Ekhzaimy, Aishah A; Masood, Afshan; Benabdelkamel, Hicham; Elhassan, Tasnem; Musambil, Mohthash; Alfadda, Assim A
• Is Part Of: Diabetes & vascular disease research. Volume 19:Number 3(2022)
• Publication Date: 2022-05
• Subjects: Liraglutide -- GLP-1 receptors agonist -- proteomics -- type 2 diabetes mellitus -- S-adenosyl homocysteine -- beta-2-glycoprotein; Diabetic angiopathies -- Periodicals; 616.462005
• DOI: 10.1177/14791641221094322
• Languages: English
• ISSNs: 1479-1641

Abstract

Background: Diabetes mellitus is a chronic multisystem disease with a high global prevalence, including in Saudi Arabia. The Glucagon-like Peptide (GLP-1) receptor agonist liraglutide is known to lower glucose levels, reduce weight and improve cardiovascular outcome. However, mechanisms underlying the benefits of liraglutide treatment in patients with type 2 diabetes mellitus (T2DM) remain unclear. Methods: In the present study, a 2D-DIGE MALDI-TOF mass spectrometric approach combined with bioinformatics and network pathway analysis explore the plasma proteomic profile. The study involved 20 patients with T2DM with mean age of 54.4 ± 9.5 years and Hemoglobin A1c (HbA1c) between 8% and 11% (inclusive). Results: A statistically significant change ( p < .006) was observed in HbA1c with no significant changes in body weight, renal function, or markers of dyslipidemia post-treatment with liraglutide. 2 D-DIGE gel analysis identified significant changes (⩾1.5-fold change, Analysis of variance (ANOVA), p ⩽ 0.05) in 72 proteins, (62 down and 10 up) in liraglutide pre-treatment compared to the post-treatment state. Proteins identified in our study were found to regulate metabolic processes including acute phase response proteins, enzymes, apolipoproteins with involvement of the inflammatory signaling pathways, NF-κB, AKT, and p38 MAPK Conclusion: Liraglutide treatment decreased levels of acute phase response that to reduce the systemic chronic inflammatory state and oxidative stress, and eventually improve the cardio-metabolic profile in these patients.