PNA microprobe for label-free detection of expanded trinucleotide repeats. Issue 13 (10th March 2022)

• Record Type: Journal Article
• Authors: Asefifeyzabadi, Narges; Durocher, Grace; Tshilenge, Kizito-Tshitoko; Alam, Tanimul; Ellerby, Lisa M.; Shamsi, Mohtashim H.
• Is Part Of: RSC advances. Volume 12:Issue 13(2022)
• Page Start: 7757
• Page End: 7761
• Publication Date: 2022-03-10
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/d2ra00230b
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : PNA-microprobe biosensing platform for label-free impedance-based detection of neurodegenerative repeat expansions. Abstract : We present a PNA microprobe sensing platform to detect trinucleotide repeat mutation by electrochemical impedance spectroscopy. The microprobe platform discriminated Huntington's disease-associated CAG repeats in cell-derived total RNA with S/N 1 : 3. This sensitive, label-free, and PCR-free detection strategy may be employed in the future to develop biosensing platforms for the detection of a plethora of repeat expansion disorders.