First report of association of Ca. Phytoplasma australasia‐related strain (16SrII‐D) with Thuja occidentalis plants showing leaf yellowing symptoms in Uttarakhand province, India. Issue 6 (20th February 2022)

• Record Type: Journal Article
• Authors: Mitra, Surabhi; Reddy, Madem G.; Rao, Govind P.
• Is Part Of: Journal of phytopathology. Volume 170:Issue 6(2022)
• Page Start: 359
• Page End: 362
• Publication Date: 2022-02-20
• Subjects: 16S rRNA gene -- 16SrII‐D subgroup -- In silico RFLP -- secA gene -- White cedar; Plant diseases -- Periodicals; 632
• DOI: 10.1111/jph.13090
• Languages: English
• ISSNs: 0931-1785

Abstract

Abstract: Thuja occidentalis L. is an evergreen coniferous species of high medicinal value with fan‐like branches and scaly leaves. Leaf yellowing symptoms were recorded on T . occidentalis plants in 2019 at Mukteshwar, Uttarakhand, India. Using the universal 16S rRNA and sec A gene phytoplasma‐specific primers, the expected amplicons of ~1.25 kb and 480 bp were consistently obtained in ten symptomatic thuja leaf samples. Virtual RFLP profiles showed that the thuja phytoplasma strains were associated with Ca . Phytoplasma australasia (16SrII‐D subgroup)‐related strain by matching the restriction patterns of the 16S rRNA gene. This is the first time a phytoplasma from the 16SrII‐D subgroup has been recorded with thuja yellows disease in the world.