Smart wound dressing based on κ–carrageenan/locust bean gum/cranberry extract for monitoring bacterial infections. (15th February 2019)

• Record Type: Journal Article
• Authors: Zepon, Karine Modolon; Martins, Maryane Modolon; Marques, Morgana Souza; Heckler, Julia Maia; Dal Pont Morisso, Fernando; Moreira, Marina Griebeler; Ziulkoski, Ana Luiza; Kanis, Luiz Alberto
• Is Part Of: Carbohydrate polymers. Volume 206(2019)
• Page Start: 362
• Page End: 370
• Publication Date: 2019-02-15
• Subjects: kappa-carrageenan (PubChem CID: 11966249) -- Citric acid (PubChem CID: 311) -- Glycerol (PubChem CID: 753); Smart wound dressing -- kappa-carrageenan -- Locust bean gum -- Cranberry -- Anthocyanins -- pH sensor; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2018.11.014
• Languages: English
• ISSNs: 0144-8617

Abstract

Graphical abstract: Highlights: κ–carrageenan/locust bean gum/cranberry hydrogel film (named κC:LBG:CB) was prepared. The in vitro pH variation could be monitored by the color change of the κC:LBG:CB hydrogel film. The presence of CB extract can inhibit bacteria growth on the κC:LBG:CB hydrogel film surface. The κC:LBG:CB hydrogel film can be used for the earlier detection of wound bacterial infection. Abstract: A smart wound dressing based on carrageenan (κC), locust bean gum (LBG), and cranberry extract (CB) for monitoring bacterial wound infections was developed and characterized using UV–vis spectroscopy, FT-IR, and SEM. The mechanical, swelling, cytotoxic and pH sensor properties were also investigated. UV–vis spectra demonstrated that the obtained κC:LBG:CB hydrogel film exhibited a visible change of colors as it was immersed in PBS solution pH 5.0, 7.3 and 9.0. The spectra of FT-IR suggested that chemical interactions had occurred between κC and CB extract. The obtained κC:LBG:CB hydrogel film exhibited adequate mechanical properties and a swelling behavior dependent on pH. Cytotoxicity tests indicated that κC:LBG:CB hydrogel film had dose-dependent cytotoxicity against NIH 3T3 fibroblast cells. The in vitro studies using Staphylococcus aureus and Pseudomonas aeruginosa demonstrated that the color changes of the κC:LBG:CB hydrogel film could be observed by naked eyes, confirming the potential use of the obtained hydrogel film as a visual system for monitoring bacterial wound infections.