Solution Viscosity‐Mediated Structural Control of Nanofibrous Sponge for RNA Separation and Purification. (9th February 2022)

• Record Type: Journal Article
• Authors: Cheng, Pan; Liu, Ke; Wan, Yucai; Hu, Wei; Ji, Cancan; Huang, Peng; Guo, Qihao; Xu, Jia; Cheng, Qin; Wang, Dong
• Is Part Of: Advanced functional materials. Volume 32:Number 20(2022)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2022-02-09
• Subjects: nanofibers -- porous sponge -- RNA adsorption -- solution viscosity -- structural control; Materials -- Periodicals; Chemical vapor deposition -- Periodicals; 620.11
• DOI: 10.1002/adfm.202112023
• Languages: English
• ISSNs: 1616-301X

Abstract

Abstract: Engineering 3D nanofibrous monolithic materials with a controllable porous channel structure and chemical activity is promising for the rapid and efficient separation of environmentally sensitive biomacromolecules such as RNA. Herein, freeze‐drying method via regulating the viscosity of nanofiber dispersions is developed to fabricate nanofibrous sponges (NFSs) with 3D structures of microsphere, lamella, and honeycomb by combining poly(vinyl alcohol‐ co ‐ethylene) (PVA‐ co ‐PE) nanofibers with chitosan (CS) and polyethyleneimine (PEI) in dispersions. Among sponges, a honeycomb nanofibrous sponge (HNFS) possesses highly oriented open‐cells and optimal performance, including water permeability of 7.431 × 10 3 L h m −2 at 1 psi, static saturated capacity of 887.6 mg g −1, dynamic capacity of 763.2 mg g −1 at a flow rate of 1 mm min −1, and mechanical robustness, superior to reported state‐of‐the‐art ion‐exchange chromatography materials. Furthermore, HNFS‐packed chromatographic, funnel, and centrifugal columns present exceptional performance and broad applicability in rapid‐separation of RNA from model solution and diverse organisms without degradation. They are attributed to the highly oriented open‐cell structure and high‐density adsorption ligands on the surface of nanofibrous cell walls. This work provides a facile approach to design advanced NFSs with versatile physicochemical structure and outstanding performance. Abstract : A strategy based on freeze‐drying via regulating the viscosity of nanofiber dispersions is developed to fabricate nanofibrous sponges with 3D structures of microsphere, lamella, and honeycomb to efficiently separate and purify RNA. This study may provide a new avenue to fabricate advanced nanofiber‐based sponges with a versatile physicochemical structure and outstanding performance.