Trehalose coated nanocellulose to inhibit the infections by S. aureus. Issue 11 (2nd February 2022)
- Record Type:
- Journal Article
- Title:
- Trehalose coated nanocellulose to inhibit the infections by S. aureus. Issue 11 (2nd February 2022)
- Main Title:
- Trehalose coated nanocellulose to inhibit the infections by S. aureus
- Authors:
- Li, Yimeng
Milewska, Małgorzata
Khine, Yee Yee
Ariotti, Nicholas
Stenzel, Martina H. - Abstract:
- Abstract : Preventing bacterial infection by using anti-adhesive trehalose polymer is one alternative to antibiotic treatment. Abstract : Preventing bacterial infection by using anti-adhesive compounds is one alternative to antibiotic treatment. Trehalose based polymers can serve as an anti-adhesive agent that are selective to bacteria as trehalose is widely used by bacteria, but there is no mechanism in mammals that processed this disaccharide. To generate an efficient trehalose-based anti-adhesive, we modified TEMPO-oxidized cellulose nanofibers (CNFs) using aldehyde-terminated trehalose polymer poly(6- O -acryloyl-trehalose) (PTre), which were prepared by RAFT polymerization, via Passerini reactions. The grafting efficiency was found to be around 35% w/w resulting in CNF-PTre with good dispersibility in water. Moreover, a control based on poly(2-hydroxy ethyl) acrylate PHEA was prepared. Both functional CNFs were non-toxic against to RAW 264.7 at concentration up to 500 μg mL −1 . The anti-adhesion effects of both functional CNFs were evaluated at concertation of 100 and 200 μg mL −1, using the S. aureus -HUVECs infection system. The treatments of CNF-PTre at 200 μg mL −1 led to a significant infection reduction of 80% while CNF-PHEA at 200 μg mL −1 decreased infections by 54% suggesting the CNF can act as anti-adhesive agent by simple steric stabilization. To generate a nanoparticle that is capable of inhibiting adhesion while as reducing bacteria growth, ciprofloxacinAbstract : Preventing bacterial infection by using anti-adhesive trehalose polymer is one alternative to antibiotic treatment. Abstract : Preventing bacterial infection by using anti-adhesive compounds is one alternative to antibiotic treatment. Trehalose based polymers can serve as an anti-adhesive agent that are selective to bacteria as trehalose is widely used by bacteria, but there is no mechanism in mammals that processed this disaccharide. To generate an efficient trehalose-based anti-adhesive, we modified TEMPO-oxidized cellulose nanofibers (CNFs) using aldehyde-terminated trehalose polymer poly(6- O -acryloyl-trehalose) (PTre), which were prepared by RAFT polymerization, via Passerini reactions. The grafting efficiency was found to be around 35% w/w resulting in CNF-PTre with good dispersibility in water. Moreover, a control based on poly(2-hydroxy ethyl) acrylate PHEA was prepared. Both functional CNFs were non-toxic against to RAW 264.7 at concentration up to 500 μg mL −1 . The anti-adhesion effects of both functional CNFs were evaluated at concertation of 100 and 200 μg mL −1, using the S. aureus -HUVECs infection system. The treatments of CNF-PTre at 200 μg mL −1 led to a significant infection reduction of 80% while CNF-PHEA at 200 μg mL −1 decreased infections by 54% suggesting the CNF can act as anti-adhesive agent by simple steric stabilization. To generate a nanoparticle that is capable of inhibiting adhesion while as reducing bacteria growth, ciprofloxacin was loaded onto CNF-PTre by ionic interaction. The resulting CNF-PTre-Cip showed an antibacterial activity against S. aureus and P. aeruginosa at a same level of free ciprofloxacin, indicating the successful release of loaded ciprofloxacin. Analysis of the rate of release at pH 7.4 and pH 5.5 showed a faster release at pH 7.4 and the drug was released in a burst-like fashion in only a few hours. … (more)
- Is Part Of:
- Polymer chemistry. Volume 13:Issue 11(2022)
- Journal:
- Polymer chemistry
- Issue:
- Volume 13:Issue 11(2022)
- Issue Display:
- Volume 13, Issue 11 (2022)
- Year:
- 2022
- Volume:
- 13
- Issue:
- 11
- Issue Sort Value:
- 2022-0013-0011-0000
- Page Start:
- 1502
- Page End:
- 1509
- Publication Date:
- 2022-02-02
- Subjects:
- Polymers -- Periodicals
Macromolecules -- Periodicals
Polymerization -- Periodicals
547.705 - Journal URLs:
- http://www.rsc.org/Publishing/Journals/PY/Index.asp ↗
http://www.rsc.org/ ↗ - DOI:
- 10.1039/d1py01422f ↗
- Languages:
- English
- ISSNs:
- 1759-9954
- Deposit Type:
- Legaldeposit
- View Content:
- Available online (eLD content is only available in our Reading Rooms) ↗
- Physical Locations:
- British Library DSC - 6547.703400
British Library DSC - BLDSS-3PM
British Library STI - ELD Digital store - Ingest File:
- 21483.xml