Correlation between CD163 expression and resting pain in patients with hip osteoarthritis: Possible contribution of CD163+ monocytes/macrophages to pain pathogenesis. Issue 6 (15th August 2021)

• Record Type: Journal Article
• Authors: Ohashi, Yoshihisa; Uchida, Kentaro; Fukushima, Kensuke; Satoh, Masashi; Koyama, Tomohisa; Tsuchiya, Maho; Saito, Hiroki; Uchiyama, Katsufumi; Takahira, Naonobu; Inoue, Gen; Takaso, Masashi
• Is Part Of: Journal of orthopaedic research. Volume 40:Issue 6(2022)
• Page Start: 1365
• Page End: 1374
• Publication Date: 2021-08-15
• Subjects: CD163+ cells -- hip osteoarthritis -- resting pain -- tumor necrosis factor‐alpha; Orthopedics -- Periodicals; Musculoskeletal system -- Periodicals; 616.7
• DOI: 10.1002/jor.25157
• Languages: English
• ISSNs: 0736-0266

Abstract

Abstract: Expression of CD163, a scavenger receptor specifically expressed by monocytes and macrophages, is elevated in the synovial tissue of patients with knee osteoarthritis (OA) compared with healthy controls. However, the association between CD163 expression in the synovium and pain in OA patients is unclear. We investigated the correlation between synovial CD163 expression and resting and active pain levels in patients with hip osteoarthritis (HOA). To investigate the possible contribution of CD163+ subsets to pain pathogenesis, we compared pain‐related cytokine expression and M1/M2 macrophage marker expression in CD163+ and CD163− cells. We performed flow cytometric analysis to study the CD163+ cell population. We also examined pain‐related cytokine expression and M1/M2 macrophage marker expression on CD163+ CD14 high and CD163+ CD14 low cells using cell sorting. Synovial CD163 expression significantly correlated with resting pain levels ( p = 0.006; R = 0.321), but not active pain levels ( p = 0.155; R = 0.169). Expression of the M1 macrophage marker CD80 was significantly higher in CD163+ than CD163− cells ( p = 0.010), as was the expression of M2 macrophage markers CD206 and IL10 ( CD206, p = 0.014; IL10, p = 0.005), and TNFA and IL1B ( TNFA, p = 0.002; IL1B, p = 0.001). TNFA expression was significantly higher in CD163+ CD14 low than CD163+ CD14 high cells, while IL1B, IL10, and CD206 expression were comparable among these subsets. Our findings suggest that CD163 expression is associated with higher resting pain scores. As TNF‐ α plays a role in the pain process, CD163+ CD14 low cells expressing TNFA may be a potent contributor to the pathogenesis of resting pain in HOA. Abstract : We investigated the correlation between synovial expression of CD163 and resting and active pain levels in patients with hip osteoarthritis (HOA), and inflammatory cell subsets among CD163+ cells. CD163 expression was significantly correlated with resting pain levels. TNFA expression was significantly higher in the CD163+ CD14 low than CD163+ CD14 high subset. As TNF‐ α plays a role in the pain process, CD163+ CD14 low cells expressing TNFA may be a potent contributor to the pathogenesis of resting pain in HOA.