Comprehensive analysis identified a reduction in ATP1A2 mediated by ARID3A in abdominal aortic aneurysm. Issue 10 (19th April 2022)

• Record Type: Journal Article
• Authors: Wang, Qunhui; Li, Na; Guo, Xian; Huo, Bo; Li, Rui; Feng, Xin; Fang, Zemin; Zhu, Xue‐Hai; Wang, Yixiang; Yi, Xin; Wei, Xiang; Jiang, Ding‐Sheng
• Is Part Of: Journal of cellular and molecular medicine. Volume 26:Issue 10(2022)
• Page Start: 2866
• Page End: 2880
• Publication Date: 2022-04-19
• Subjects: abdominal aortic aneurysm -- ARID3A -- ATP1A2 -- blood pressure -- VSMCs; Cytology; Medicine; Molecular Biology; Cytologie -- Périodiques; Médecine -- Périodiques; Biologie moléculaire -- Périodiques; Cytology -- Periodicals; Medicine -- Periodicals; Molecular biology -- Periodicals; 611.01805
• DOI: 10.1111/jcmm.17301
• Languages: English
• ISSNs: 1582-1838

Abstract

Abstract: Abdominal aortic aneurysm (AAA) is characterized by abdominal aorta dilatation and progressive structural impairment and is usually an asymptomatic and potentially lethal disease with a risk of rupture. To investigate the underlying mechanisms of AAA initiation and progression, seven AAA datasets related to human and mice were downloaded from the GEO database and reanalysed in the present study. After comprehensive bioinformatics analysis, we identified the enriched pathways associated with inflammation responses, vascular smooth muscle cell (VSMC) phenotype switching and cytokine secretion in AAA. Most importantly, we identified ATPase Na + /K + transporting subunit alpha 2 (ATP1A2) as a key gene that was significantly decreased in AAA samples of both human and mice; meanwhile, its reduction mainly occurred in VSMCs of the aorta; this finding was validated by immunostaining and Western blot in human and mouse AAA samples. Furthermore, we explored the potential upstream transcription factors (TFs) that regulate ATP1A2 expression. We found that the TF AT‐rich interaction domain 3A (ARID3A) bound the promoter of ATP1A2 to suppress its expression. Our present study identified the ARID3A‐ATP1A2 axis as a novel pathway in the pathological processes of AAA, further elucidating the molecular mechanism of AAA and providing potential therapeutic targets for AAA.