Front Cover: Generic reversed‐phase ultra‐high‐pressure liquid chromatography methodology developed by using computer‐assisted modeling for streamlined performance evaluation of a wide range of stationary phase columns. Issue 5 (11th May 2022)

• Record Type: Journal Article
• Authors: Ahmad, Imad A. Haidar; Losacco, Gioacchino Luca; Figus, Margaret; Korani, Deepa; Gunsch, Matthew J.; Lhotka, Hayley R.; Hullen, Kari; Hartman, Robert; Lohani, Sachin; Hamilton, Simon; Mangion, Ian; Regalado, Erik L.
• Is Part Of: Separation science plus. Volume 5:Issue 5(2022)
• Page Start: NA
• Page End: NA
• Publication Date: 2022-05-11
• Subjects: Chromatographic analysis -- Periodicals; 543.8
• DOI: 10.1002/sscp.202270031
• Languages: English
• ISSNs: 2573-1815

Abstract

Abstract : DOI: 10.1002/sscp.202200002 A generic probe methodology was developed using in silico chromatography modeling tools. The separation of a standard multicomponent mixture was optimized on multiple columns/mobile phases. An optimal space across the overlay of different resolution maps is identified and used as a generic probe condition under which the components of the standard mixture will be resolved on all columns/mobile phases combinations. The resulting generic probe is used as a framework for testing column robustness and stability to ensure a straightforward method development and transfer workflow.