Back Cover: Smart proteolysis samplers for pre‐lab bottom‐up protein analysis – Performance of on‐paper digestion compared to conventional digestion. Issue 5 (11th May 2022)

• Record Type: Journal Article
• Authors: Nguyen, Minh Thao; Halvorsen, Trine Grønhaug; Thiede, Bernd; Reubsaet, Léon
• Is Part Of: Separation science plus. Volume 5:Issue 5(2022)
• Page Start: NA
• Page End: NA
• Publication Date: 2022-05-11
• Subjects: Chromatographic analysis -- Periodicals; 543.8
• DOI: 10.1002/sscp.202270036
• Languages: English
• ISSNs: 2573-1815

Abstract

Abstract : DOI: 10.1002/sscp.202100062 The cover picture shows filter paper which is converted into a sample preparation unit for high‐end bottom‐up protein analysis. The trypsin covalently bound to cellulose digests proteins enabling shotgun and targeted proteomics experiments from dried matrix spots. These paper‐based smart proteolysis samplers allow instant sample preparation starting at the moment of sampling with potential continuation during postal transport. The smart proteolysis sampler shows good stability (no activity loss for at least 4 months when refrigerated) and the proteolytic activity of bound trypsin is up to 50–60% of trypsin acting in solution