Neutrophil extracellular traps are associated with enhanced procoagulant activity in liver cirrhosis patients with portal vein thrombosis. Issue 5 (18th April 2022)

• Record Type: Journal Article
• Authors: Xing, Yueyi; Jiang, Yueping; Xing, Shichao; Mao, Tao; Guan, Ge; Niu, Qinghui; Zhao, Xianzhi; Zhou, Jianrui; Jing, Xue
• Is Part Of: Journal of clinical laboratory analysis. Volume 36:Issue 5(2022)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2022-04-18
• Subjects: hypercoagulability -- liver cirrhosis -- neutrophil extracellular traps -- portal vein thrombosis; Diagnosis, Laboratory -- Periodicals; Medical laboratory technology -- Periodicals; 616
• DOI: 10.1002/jcla.24433
• Languages: English
• ISSNs: 0887-8013

Abstract

Abstract: Objective: Patients with liver cirrhosis (LC) commonly exhibit hypercoagulability and tend to develop thrombosis. Neutrophil extracellular traps (NETs) are associated with a variety of thrombotic conditions, but their possible value in portal vein thrombosis (PVT) is not known. We assessed whether NETs promote thrombosis and contribute to the procoagulant state in patients with LC. Methods: The circulating levels of NETs markers (myeloperoxidase, neutrophil elastase, citrullinated histone H3) were measured in 72 patients (median age, 55 years; 48 [66.7%] men) with LC from September 2020 to February 2021. Then they were divided into two groups: patients with or without PVT. NETs procoagulant activity was assessed based on thrombin–antithrombin complex (TAT complex) and Factor X. The levels of plasma markers were determined by ELISA. Results: There were 28 patients with PVT and 44 patients without PVT. The levels of NETs markers and hypercoagulability markers in the plasma of cirrhosis patients with PVT were significantly higher than those of cirrhosis patients without PVT ( p < 0.05). Additionally, the levels of the NETs markers correlated with TAT complex and Factor X (Spearman correlation rho >0.73, p < 0.0001). Conclusions: Neutrophil extracellular traps seem to enhance procoagulant activity in LC patients with PVT; thus, they may be a practical predictor of PVT as well as a rapid and easy‐to‐use diagnostic and treatment guide for PVT in patients with cirrhosis. Abstract : Patients with PVT had higher serum NETs markers levels compared with non‐PVT patients. Levels of MPO, NE, and CitH3 in the PVT group ( n = 28) were significantly higher than those in the non‐PVT group ( n = 44). Levels of MPO were positively associated with circulating levels of NE and CitH3 in PVT patients. **: p < 0.01; *: p < 0.05.