(±)-Equol interferes with the secretion of gonadotropin-releasing hormone and the expression of neuromodulators in murine neurons. Issue 1 (2nd January 2022)

• Record Type: Journal Article
• Authors: Xiong, Jingyuan; Wang, Minghui; Tian, Ye; Ling, Aru; Shan, Shufang; Zhao, Li; Cheng, Guo
• Is Part Of: Toxicological and environmental chemistry. Volume 104:Issue 1(2022)
• Page Start: 129
• Page End: 140
• Publication Date: 2022-01-02
• Subjects: Equol -- gonadotropin-releasing hormone -- hypothalamic-pituitary-gonadal axis -- isoflavones -- phytoestrogen; neuroendocrinology; Toxicology -- Periodicals; Environmental chemistry -- Periodicals; 571.95
• DOI: 10.1080/02772248.2022.2055031
• Languages: English
• ISSNs: 0277-2248

Abstract

Abstract: S-Equol is an intestinal bacterial metabolite of the isoflavone daidzein. Racemic (± )-equol is used as a dietary estrogen supplement in menopausal women and is suggested to affect the female reproductive function and development, primarily controlled by the hypothalamic-pituitary-gonadal axis and gonadotropin-releasing hormone release. In this study, murine neurons were utilized to evaluate the effect and mechanism of (± )-equol on the secretion of gonadotropin-releasing hormone. The cells were treated with up to 20 µmol/L of (± )-equol to determine the influence on cell viability. Enzyme-linked immunosorbent assay, real-time quantitative polymerase chain reaction, and Western-blotting analysis were used to investigate the impacts of (± )-equol on the secretion and transcription of gonadotropin-releasing hormone, and the transcription and translation of key neuromodulators. This study showed that (± )-equol could decrease cell viability, increase the ability to secrete gonadotropin-releasing hormone, and interfere with the expression of neurokinin B receptor, makorin ring finger protein 3, and silent information regulator 1.