Cell Microarrays: An Emerging Technology for the Characterization of Antibodies. (December 2002)

• Record Type: Journal Article
• Authors: Schwenk, Jochen M.; Stoll, Dieter; Templin, Markus F.; Joos, Thomas O.
• Is Part Of: Biotechniques. Volume 33(2002)Supplement 6
• Page Start: S54
• Page End: S61
• Publication Date: 2002-12
• Subjects: Biology, Experimental -- Periodicals; Molecular biology -- Periodicals; Medical technology -- Periodicals; Biology, Experimental; Medical technology; Molecular biology; Clinical Laboratory Techniques -- Periodicals; Research -- Periodicals; Medical Laboratory Science -- Periodicals; Periodicals; Electronic journals; 570
• DOI: 10.2144/dec02schwenk
• Languages: English
• ISSNs: 0736-6205

Abstract

The possibility to miniaturize and parallelize biological assays has a great impact on the development of biomedical technologies. Here, we describe a simple, miniaturized, and parallelized method employing entire cells from different cell lines displaying a protein of interest on their surface, which were immobilized on a microarray slide. Antibodies were added to these cellular microarrays, and their specific binding to the cell surface proteins was monitored using appropriate fluorescently labeled detection molecules. This new method is applicable for rapidly screening cell surface-specific antibodies with respect to selectivity and cross-reactivity.