Automated Screening Procedure for High-Throughput Generation of Antibody Fragments. (December 2002)

• Record Type: Journal Article
• Authors: Hallborn, Johan; Carlsson, Roland
• Is Part Of: Biotechniques. Volume 33(2002)Supplement 6
• Page Start: S30
• Page End: S37
• Publication Date: 2002-12
• Subjects: Biology, Experimental -- Periodicals; Molecular biology -- Periodicals; Medical technology -- Periodicals; Biology, Experimental; Medical technology; Molecular biology; Clinical Laboratory Techniques -- Periodicals; Research -- Periodicals; Medical Laboratory Science -- Periodicals; Periodicals; Electronic journals; 570
• DOI: 10.2144/dec02-hallborn
• Languages: English
• ISSNs: 0736-6205

Abstract

In the emerging field of proteomics, there is an urgent need for catcher molecules such as antibodies for detecting the proteome or parts of the proteome in a microarray format. A suitable source for providing a large diversity of binders is obtained by combinatorial libraries, such as phage display libraries of single chain antibody fragments (scFv) or Fab fragments. To find novel binders from the n-CoDeR libraries with a high throughput, we have automated the screening process with robotics. The automated system is configured to screen tens of thousands of clones per day to target antigens in various formats, including peptides and soluble proteins, as well as cell-bound targets; thus, it is well designed to meet demands from the proteomics area.