Analysis of three emulsifiers of diglyceride in milk by ultra performance liquid chromatography – Evaporative light scattering detector. Issue 9 (15th June 2021)

• Record Type: Journal Article
• Authors: Luo, Yun; Xu, Ying; Yu, Xuan; Yan, Aiping; Lan, Guo; Liu, Fan; Wan, Yiqun
• Is Part Of: Journal of liquid chromatography & related technologies. Volume 44:Issue 9/10(2021)
• Page Start: 501
• Page End: 506
• Publication Date: 2021-06-15
• Subjects: 1, 3-Dipalmitate glyceride -- glyceryl 1, 3-distearate -- glyceryl dilinoleate -- liquid chromatography-evaporative light scattering -- milk -- ultra performance; Liquid chromatography -- Periodicals; Chromatographic analysis -- Periodicals; Separation (Technology) -- Periodicals; Biotechnology -- Periodicals; Chromatography, Liquid -- Periodicals; 543.8405
• DOI: 10.1080/10826076.2021.1968428
• Languages: English
• ISSNs: 1082-6076

Abstract

Abstract: In this paper, a new method was developed for the determination of glyceryl dilinoleate, 1, 3-dipalmitate glyceride, and glyceryl 1, 3-distearate in milk by ultra-performance liquid chromatography—evaporative light scattering detector (UPLC-ELSD). The analytes were extracted with acetone, dried by nitrogen and then dissolved in isopropanol. Under the optimized conditions, glyceryl dilinoleate (10–100 mg/L), glyceryl 1, 3-dipalmitate (10–100 mg/L) and glyceryl 1, 3-distearate (10–100 mg/L) showed a good linear relationship ( R 2 >0.997), with LODs and LOQs in the range of 3.15–3.19 mg/L and 10.30–10.50 mg/L, respectively. The recoveries of the analytes in the milk were in the range of 72.5% − 127.5%, with the relative standard deviations (RSD) ( n = 6) less than 6.0%. All the results indicated that the method was suitable for the determination of glyceryl dilinoleate, glyceryl 1, 3-dipalmitate and glyceryl 1, 3-distearate in milk. Graphical Abstract: UF0001