Arabidopsis exoribonuclease USB1 interacts with the PPR-domain protein SOAR1 to negatively regulate abscisic acid signaling. (24th September 2020)

• Record Type: Journal Article
• Authors: Ma, Yu; Zhang, Shang; Bi, Chao; Mei, Chao; Jiang, Shang-Chuan; Wang, Xiao-Fang; Lu, Zhi John; Zhang, Da-Peng
• Editors: Spoel, Steven
• Is Part Of: Journal of experimental botany. Volume 71:Number 19(2020)
• Page Start: 5837
• Page End: 5851
• Publication Date: 2020-09-24
• Subjects: Abscisic acid signaling -- Arabidopsis thaliana -- early seedling growth -- exoribonuclease USB1 -- pentatricopeptide repeat (PPR) protein SOAR1 -- spliceosome assembly; Botany -- Periodicals; Botany, Experimental -- Periodicals; Plant physiology -- Periodicals; 580
• DOI: 10.1093/jxb/eraa315
• Languages: English
• ISSNs: 0022-0957

Abstract

Abstract : USB1, an exoribonuclease catalyzing U6 production for spliceosome assembly, negatively regulates, cooperatively with SOAR1, ABA signaling in early seedling development, probably by affecting spliceosome assembly. Abstract: Signaling by the phytohormone abscisic acid (ABA) involves pre-mRNA splicing, a key process of post-transcriptional regulation of gene expression. However, the regulatory mechanism of alternative pre-mRNA splicing in ABA signaling remains largely unknown. We previously identified a pentatricopeptide repeat protein SOAR1 (suppressor of the ABAR-overexpressor 1) as a crucial player downstream of ABAR (putative ABA receptor) in ABA signaling. In this study, we identified a SOAR1 interaction partner USB1, which is an exoribonuclease catalyzing U6 production for spliceosome assembly. We reveal that together USB1 and SOAR1 negatively regulate ABA signaling in early seedling development. USB1 and SOAR1 are both required for the splicing of transcripts of numerous genes, including those involved in ABA signaling pathways, suggesting that USB1 and SOAR1 collaborate to regulate ABA signaling by affecting spliceosome assembly. These findings provide important new insights into the mechanistic control of alternative pre-mRNA splicing in the regulation of ABA-mediated plant responses to environmental cues.