Molecular Identification of Fasciola Isolated from the Liver of Meat Animals in Fars Province, Iran. (26th March 2022)

• Record Type: Journal Article
• Authors: Saadatnia, Aminallah; Solhjoo, Kavous; Davami, Mohamad Hasan; Raeghi, Saber; Abolghazi, Ahmad
• Other Names: López-Arellano María Eugenia Academic Editor.
• Is Part Of: Journal of parasitology research. Volume 2022(2022)
• Publication Date: 2022-03-26
• Subjects: Parasitology -- Periodicals; Parasitology -- Research -- Periodicals; Parasitic Diseases; Parasitology; Parasitology; Parasitology -- Research; Electronic journals; Periodicals; Periodicals; 616.96
• DOI: 10.1155/2022/4291230
• Languages: English
• ISSNs: 2090-0023

Abstract

Abstract : Background . Fasciola hepatica and Fasciola gigantica are flatworms that infect animals and humans. Fasciola is the parasite of the liver or bile ducts and intestines of mammals, where such animals are known as their "definite hosts." The study aims to detect the genotype of Fasciola spp. from the livers of meat animals by using RFLP-PCR in samples collected from Fars province. Methods . Sixty Fasciola spp. samples were collected from infected slaughtered animals in three counties of Fars province, Iran (Jahrom, Nourabad Mamasani, and Kazeroun).Genomic DNA was extracted by the conventional phenol-chloroform method. For the study, PCR-RFLP and sequence analysis of the first nuclear ribosomal internal transcribed spacer (ITS1) region from Fasciola species were used to conduct the study. Results . The fragment of about 700 bp in all the Fasciola samples was amplified. In total, 43 samples of Fasciola gigantica and 17 samples of Fasciola hepatica were identified. Conclusion . The dominant Fasciola species in this region is Fasciola gigantica . Hence, it seems that hygienic policies should be developed to prevent and control fascioliasis because of the dominant species, Fasciola gigantica .