A Computational model of the mammalian external tufted cell. (7th February 2019)

• Record Type: Journal Article
• Authors: Viertel, Ryan; Borisyuk, Alla
• Is Part Of: Journal of theoretical biology. Volume 462(2019)
• Page Start: 109
• Page End: 121
• Publication Date: 2019-02-07
• Subjects: External tufted cell -- Bursting -- Glomerulus -- Olfactory bulb -- Hodgkin Huxley model; 37N25; Biology -- Periodicals; Biological Science Disciplines -- Periodicals; Biology -- Periodicals; Biologie -- Périodiques; Theoretische biologie; Biology; Periodicals; 571.05
• DOI: 10.1016/j.jtbi.2018.10.003
• Languages: English
• ISSNs: 0022-5193

Abstract

Highlights: We create and verify a biophysically-detailed model of the olfactory blub external tufted cell. Depolarizing (but not hyperpolaryzing) inputs received during the inter-burst interval can advance burst timing. Specific currents controlling each burst characteristic are identified. A heterogeneous population produces reliable responses to a range of periodic inputs, even though individual cells are entrained by inputs with higher, rather than lower frequencies. Abstract: We introduce a novel detailed conductance-based model of the bursting activity in external tufted (ET) cells of the olfactory bulb. We investigate the mechanisms underlying their bursting, and make experimentally-testable predictions. The ionic currents included in the model are specific to ET cells, and their kinetic and other parameters are based on experimental recordings. We validate the model by showing that its bursting characteristics under various conditions (e.g. blocking various currents) are consistent with experimental observations. Further, we identify the bifurcation structure and dynamics that explain bursting behavior. This analysis allows us to make predictions of the response of the cell to current pulses at different burst phases. We find that depolarizing (but not hyperpolarizing) inputs received during the interburst interval can advance burst timing, creating the substrate for synchronization by excitatory connections. It has been hypothesized that such synchronization among the ET cells within one glomerulus might help coordinate the glomerular output. Next we investigate model parameter sensitivity and identify parameters that play the most prominent role in controlling each burst characteristic, such as the burst frequency and duration. Finally, the response of the cell to periodic inputs is examined, reflecting the sniffing-modulated input that these cell receive in vivo . We find that individual cells can be better entrained by inputs with higher, rather than lower, frequencies than the intrinsic bursting frequency of the cell. Nevertheless, a heterogeneous population of ET cells (as may be found in a glomerulus) is able to produce reliable periodic population responses even at lower input frequencies.