Biomass-bound biocatalysts for biodiesel production: Tuning a lipolytic activity from Aspergillus niger MYA 135 by submerged fermentation using agro-industrial raw materials and waste products. (January 2019)

• Record Type: Journal Article
• Authors: Regner, Erika Lucía; Salvatierra, Hebe Natalia; Baigorí, Mario Domingo; Pera, Licia María
• Is Part Of: Biomass and bioenergy. Volume 120(2019)
• Page Start: 59
• Page End: 67
• Publication Date: 2019-01
• Subjects: Biomass-bound biocatalysts -- Biodiesel -- Lipolytic activity -- Aspergillus niger -- Plackett-Burman design -- Morphological characterization; Biomass energy -- Periodicals; Biomass -- Periodicals; Energy-Generating Resources -- Periodicals; Bioénergie -- Périodiques; 333.9539
• DOI: 10.1016/j.biombioe.2018.11.005
• Languages: English
• ISSNs: 0961-9534

Abstract

Abstract: The enzymatic route is a promising strategy to synthesize biodiesel essentially because of its moderate reaction conditions and easy product separation. Additionally, coupling the biodegradation of agro-industrial raw materials and waste products with the enzyme production has also had a significant impact on the development of industrial biotechnology. This is mainly due to the low-cost and the prevention of pollution they represent. In this work, different kinds of residues and wastes were used for tuning biomass-bound lipolytic biocatalysts from Aspergillus niger MYA 135 by submerged fermentation using the Plackett-Burman design. As a result, by a specific combination of medium components, two distinctive biocatalysts were obtained. Thus, the production of a mycelium-bound esterase was maximized in the presence of (in g l −1 ): CaCl2 0.5, FeCl3 1.0, corn meal 2.0, cerelose 10.0, peptone 10.0, and waste cooking oil 10.0. While, the production of a mycelium-bound lipase was selectively increased in the presence of (in g l −1 ): molasses 5.0, vinasse 5.0, CaCl2 0.5, FeCl3 1.0, milk serum 10.0, cerelose 5.0, peptone 5.0 and waste cooking oil 10.0. Besides, the biomass-bound lipase activity produced in the optimized culture medium was also prepared either free or immobilized in alginate beads, and then individually placed in small-scaled packed-bed reactors to produce biodiesel compounds in a solvent-free system using soybean oil and butanol as substrates. After a three-stepwise addition of butanol, a biodiesel conversion of 57.32 and 95.89% was reached in the presence of free and immobilized biomass, respectively. Biocatalysts morphologies were also analyzed. Graphical abstract: Image 1 Highlights: Submerged fermentation is itself a powerful tool for tuning biocatalyst properties. Coupling substrates biodegradation with lypolytic enzymes production was analyzed. A specific combination of medium components yielded two different biocatalysts. Both biocatalysts displayed a coexistence of free mycelium and hairy pellets. Enzymatic packed-bed reactors were successfully developed for biodiesel production.