Dysfunction of epithelial permeability barrier induced by HMGB1 in 2.5D cultures of human epithelial cells. Issue 2 (3rd April 2022)

• Record Type: Journal Article
• Authors: Kojima, Takashi; Shindo, Yuma; Konno, Takumi; Kodera, Yuki; Arai, Wataru; Miyakawa, Maki; Ohwada, Kizuku; Tanaka, Hiroki; Tsujiwaki, Mitsuhiro; Sakuma, Yuji; Kikuchi, Shin; Ohkuni, Tsuyoshi; Takano, Kenichi; Watanabe, Atsushi; Kohno, Takayuki
• Is Part Of: Tissue barriers. Volume 10:Issue 2(2022)
• Publication Date: 2022-04-03
• Subjects: 2.5d matrigel cultures -- epithelial permeability barriers -- hmgb1 -- proinflammatory cytokines -- tight junctions -- claudins -- angulin-1/LSR -- tricellulin -- TGF-β -- EW-7197 -- PYK2 -- PF431396 -- tnfα-antibody -- p63 -- normal human pulmonary epithelial cells -- normal human nasal epithelial cells -- human intestinal epithelial cell line CACO-2 -- cell metabolism; Biological transport -- Periodicals; Tissues -- Periodicals; Biological Transport -- Periodicals; Tissues -- Periodicals; 571
• DOI: 10.1080/21688370.2021.1972760
• Languages: English
• ISSNs: 2168-8370

Abstract

ABSTRACT: Airway and intestinal epithelial permeability barriers are crucial in epithelial homeostasis. High mobility group box 1 (HMGB1), increased by various stimuli, is involved in the induction of airway inflammation, as well as the pathogenesis of inflammatory bowel disease. HMGB1 enhances epithelial hyperpermeability. Two-and-a-half dimensional (2.5D) culture assays are experimentally convenient and induce cells to form a more physiological tissue architecture than 2D culture assays for molecular transfer mechanism analysis. In 2.5D culture, treatment with HMGB1 induced permeability of FITC-dextran into the lumen formed by human lung, nasal and intestinal epithelial cells. The tricellular tight junction molecule angulin-1/LSR is responsible for the epithelial permeability barrier at tricellular contacts and contributes to various human airway and intestinal inflammatory diseases. In this review, we indicate the mechanisms including angulin-1/LSR and multiple signaling in dysfunction of the epithelial permeability barrier induced by HMGB1 in 2.5D culture of human airway and intestinal epithelial cells.