Biohydrogen production from dairy industry wastewater in an anaerobic fluidized-bed reactor. (January 2019)

• Record Type: Journal Article
• Authors: Silva, Aline Neves da; Macêdo, Williane Vieira; Sakamoto, Isabel Kimiko; Pereyra, Daniela de Los Angeles Dalmao; Mendes, Camila Oliveira; Maintinguer, Sandra Imaculada; Caffaro Filho, Roberto Augusto; Damianovic, Marcia Helena Zamariolli; Varesche, Maria Bernadete Amancio; Amorim, Eduardo Lucena Cavalcante de
• Is Part Of: Biomass and bioenergy. Volume 120(2019)
• Page Start: 257
• Page End: 264
• Publication Date: 2019-01
• Subjects: Anaerobic digestion -- Hydrogen production -- Dairy wastewater; Biomass energy -- Periodicals; Biomass -- Periodicals; Energy-Generating Resources -- Periodicals; Bioénergie -- Périodiques; 333.9539
• DOI: 10.1016/j.biombioe.2018.11.025
• Languages: English
• ISSNs: 0961-9534

Abstract

Abstract: The present study evaluated the influence of organic loading rate in biohydrogen production from dairy wastewater in an anaerobic fluidized-bed reactor inoculated by natural fermentation of the wastewater used during the operational period. The support material for biomass adhesion was shredded tire. The reactor had a working volume of 908.4 cm 3 . Biohydrogen production was evaluated using three different organic loading rates (OLR) of 28.7 kgCOD m − ³d −1, 53.2 kgCOD m − ³d −1 e 101, 7 kgCOD m − ³d −1 corresponding to the Hydraulic Retention Time (HRT) of 8 h (2.7 L d −1 ), 6 h (3.6 L d −1 ) and 4 h (5.4 L d −1 ), respectively. The hydrogen yield decreased with increasing organic loading rate (OLR) from 2.56 ± 0.62 mol H2 mol carbohydrate −1 to 0.95 ± 0.28 mol H2 mol carbohydrate −1 as the OLR increased from 28.65 kgCOD m − ³d −1 to 95.76 kgCOD m − ³d −1 . The hydrogen content in the biogas (volume of H2 / volume of biogas) produced and the highest volumetric production were respectively 35.72 ± 9.43% and 0.80 ± 0.21 LH2 h −1 L −1 obtained when the OLR was 53.25 ± 7.81 kgCOD m − ³d −1 . The main metabolites produced were acetic acid (67.15%-87.42%) and ethanol (6.01%-13.09%). Increasing the applied OLR also caused an increase in bacterial diversity as evidenced by the highest value of the Shannon-Wiener index (2.811) corresponding to the operational phase of the highest OLR (95.76 ± 29.10 kgCOD m − ³d −1 ). Highlights: Hydrogen was produced from the biological treatment of dairy industry wastewater. Organic loading rates were evaluated for H2 production. The highest hydrogen yield was obtained at the OLR of 28.7 ± 8.9 kgCOD m −3 d −1 . The main metabolites produced in the anaerobic process were acetic acid (67%-87%) and ethanol (6%-13%). It was observed an increasing trend of microbial diversity with increasing the applied OLR.