Evaluation of Toxicity in Mouse Bone Marrow Progenitor Cells. Issue 1 (1st February 2016)

• Record Type: Journal Article
• Authors: Ezeh, Peace C.; Xu, Huan; Wang, Shu Chun; Medina, Sebastian; Burchiel, Scott W.
• Editors: Costa, Lucio G; Davila, Julio C.; Lawrence, David A.; Reed, Donald J.
• Is Part Of: Current protocols in toxicology. Volume 67:Issue 1(2016)
• Page Start: 18.9.1
• Page End: 18.9.12
• Publication Date: 2016-02-01
• Subjects: bone marrow toxicology -- hematopoiesis -- progenitor cell differentiation -- CFU assays -- surface and intracellular staining; Toxicology -- Laboratory manuals; Toxicology -- methods; Toxicology; Laboratory Manual; Electronic books; Laboratory manuals; 571.95028
• DOI: 10.1002/0471140856.tx1809s67
• Languages: English
• ISSNs: 1934-9254

Abstract

Abstract: Development of blood cells through hematopoiesis occurs in the bone marrow (BM), and can be adversely impacted by various substances and/or conditions ranging from known therapeutic, intentionally administered xenobiotics to unintentional food additives and exposure to environmental chemicals. The principles underlying the techniques for evaluating toxicity to BM progenitors (erythroid, myeloid, and lymphoid) exploit changes in the normal hematopoietic process, biochemical cell surface and intracellular markers, as well as components of the BM microenvironment. Toxicological investigations following in vivo exposures of mice or in vitro exposures of mouse primary BM cell cultures allow the assessment of the developmental and functional integrity of BM cells, cell population shifts, and adverse biochemical effects due to toxicity. Colony forming unit (CFU) assays and flow cytometry are indispensable techniques in these toxicity studies. © 2016 by John Wiley & Sons, Inc.