Burkholderia thailandensis: Growth and Laboratory Maintenance. Issue 1 (12th August 2016)

• Record Type: Journal Article
• Authors: Garcia, Erin C.; Cotter, Peggy A.
• Editors: Coico, Richard; McBride, Alison; Quarles, John M.; Stevenson, Brian; Taylor, Ronald K.
• Is Part Of: Current protocols in microbiology. Volume 42:Issue 1(2016)
• Page Start: 4C.1.1
• Page End: 4C.1.7
• Publication Date: 2016-08-12
• Subjects: Burkholderia thailandensis -- Burkholderia pseudomallei -- growth -- model organism; Microbiology -- Laboratory manuals; Microbiology -- Technique -- Periodicals; Microbiological Techniques -- methods; Containment of Biohazards -- methods; Laboratory Infection -- prevention & control; Microbiology; Microbiology -- Technique; Fulltext; Internet Resources; Laboratory Manuals; Reference Materials; Periodicals; Laboratory manuals; 579.028
• DOI: 10.1002/cpmc.15
• Languages: English
• ISSNs: 1934-8525

Abstract

Abstract: Burkholderia thailandensis is a nonpathogenic Gram‐negative bacterium found in tropical soils. Closely related to several human pathogens, its ease of genetic manipulation, rapid growth in the laboratory, and low virulence make B. thailandensis a commonly used model organism. This unit describes the fundamental protocols for in vitro growth and maintenance of B. thailandensis in the laboratory. © 2016 by John Wiley & Sons, Inc.