The occurrence of Listeria monocytogenes in goats, farm environment and invertebrates. Issue 6 (3rd June 2022)

• Record Type: Journal Article
• Authors: Kulesh, Rashmi; Shinde, S. V.; Khan, W. A.; Chaudhari, S. P.; Patil, A. R.; Kurkure, N. V.; Paliwal, Neha; Likhite, Amrut V.; Zade, N.N.; Barbuddhe, Sukhadeo B
• Is Part Of: Biological rhythm research. Volume 53:Issue 6(2022)
• Page Start: 831
• Page End: 840
• Publication Date: 2022-06-03
• Subjects: Listeria monocytogenes -- Listeriolysin O -- goats -- PCR -- isolation; Biological rhythms -- Periodicals; Chronobiology -- Periodicals; Cycles -- Periodicals; Biological Clocks; Chronobiology; Circadian Rhythm; 570
• DOI: 10.1080/09291016.2019.1660836
• Languages: English
• ISSNs: 0929-1016

Abstract

ABSTRACT: The occurrence of Listeria monocytogenes at seven different organized goat farms and its environment was studied. A total of 482 samples from goats having a history of abortion and apparently healthy animals (n = 380), farm environment (n = 51) and invertebrates (n = 51; flies and ticks) were collected. Besides, serum samples were collected from goats having a history of abortion (n = 95) and apparently healthy animals (n= 98). The isolation of the pathogen was performed with two-step enrichment followed by selective plating. Listeria spp. were isolated from 26 (5.39%) samples. Of these, 12 (3.15%) isolates were confirmed as L. monocytogenes recovered from goats, 1 isolate from farm environment (soil) and 2 isolates from invertebrates (fly and tick). Out of seven organized farms screened, three showed the occurrence of L. monocytogenes in the animals having a history of abortion, associated farm environment and invertebrates. PCR-based serotyping of L. monocytogenes revealed 14 isolates to be of serogroup 4b, 4d, 4e and one of serogroup 1/2a, 1/2c, 3a, 3c. Seropositivity using indirect ELISA was observed in 11 (11.58%) goats having a history of abortion and 3(3.06%) inapparently healthy goats. This study highlighted the role of vectors in listeric infections. Abbreviations: UVM - University of Vermont Medium; PALCAM - Polymyxin Acriflavin Lithium-chloride Ceftazidime Esculin Mannitol; LLO- Listeriolysin O