Hydrogen sulfide protects retina from blue light-induced photodamage and degeneration via inhibiting ROS-mediated ER stress-CHOP apoptosis signal. (31st December 2022)

• Record Type: Journal Article
• Authors: Zhu, Sen; Li, Xuan; Dang, Bingrong; Wu, Fen; Gou, Kexin; Wang, Chunming; Lin, Changjun
• Is Part Of: Redox report. Volume 27:Number 1(2022)
• Page Start: 100
• Page End: 110
• Publication Date: 2022-12-31
• Subjects: H2S -- photodamage -- retinal degeneration -- oxidative damage -- endoplasmic reticulum stress -- reactive oxygen species‌ -- apoptosis -- ARPE-19 cells; Free radicals (Chemistry) -- Pathophysiology -- Periodicals; Oxidation, Physiological -- Periodicals; 541.224
• DOI: 10.1080/13510002.2022.2069534
• Languages: English
• ISSNs: 1351-0002

Abstract

ABSTRACT: Background: Hydrogen sulfide (H2 S) is a small reducing gas molecule with various biological functions such as anti-oxidative, anti-apoptotic and anti-inflammatory activities. In this study, we investigated the therapeutic effects of exogenous H2 S in the experimental models of retinal photodamage in vivo and in vitro. Methods: Rats with open eyelids were pretreated with H2 S (80~120 μmol/kg) for 10 days and then continuously exposed to blue light (435~445nm, 11.2W/m2) for 8 h to establish in vivo experimental model. ARPE-19 cells were pretreated with H2 S and then exposed to blue light to establish in vitro experimental model. Results: In vivo experiments, H2 S significantly ameliorated blue light-induced retinal oxidative stress, apoptosis and degeneration. Moreover, H2 S inhibited the activation of blue light-induced endoplasmic reticulum (ER) stress CHOP apoptotic signaling. In vitro experiments, H2 S improved blue light-induced oxidative stress and oxidative damage. H2 S inhibited ROS-mediated activation of ER stress CHOP apoptotic signaling. H2 S alleviated blue light-induced apoptosis and increases cell viability. The ER stress inhibitor 4-PBA alleviated blue light-induced apoptosis and increases cell viability. Conclusion: Taken together, these results indicate that H2 S can inhibit ROS-mediated ER stress-CHOP apoptosis signal, thereby alleviating blue light-triggered retinal apoptosis and degeneration.