Chitinophaga hostae sp. nov., isolated from the rhizosphere soil of Hosta plantaginea. Issue 4 (28th April 2022)

• Record Type: Journal Article
• Authors: He, Shan-Wen; Ma, Rong; Zhao, Ying-Ying; An, Lei; Huang, Jun-Hua; Zhang, Qi; Han, Ji-Gang; Zhang, Xiao-Xia
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 72:Issue 4(2022)
• Publication Date: 2022-04-28
• Subjects: Chitinophaga -- Chitinophagaceae -- Hosta plantaginea; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.005335
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : A Gram-negative, rod-shaped aerobic bacterium designated as strain 2R12 T was isolated from the rhizosphere soil of Hosta plantaginea . Phylogenetic analyses based on the 16S rRNA gene revealed that strain 2R12 T should be assigned to the genus Chitinophaga with the highest sequence similarity to Chitinophaga arvensicola DSM 3695 T (99.1 %) and Chitinophaga ginsengisegetis DSM 18108 T (98.6 %). The major fatty acids of strain 2R12 T (>10 %) were iso-C15 : 0, C16 :1 ω 5 c and iso-C17 : 0 3-OH. The major polar lipids were phosphatidylethanolamine, two unidentified aminolipids and five unidentified lipids. The predominant respiratory quinone was MK-7. The genomic DNA G+C content was 46.1 mol%. The average nucleotide identity values of strain 2R12 T with C. arvensicola DSM 3695 T and C. ginsengisegetis DSM 18108 T were 77.9 and 78.8 %, respectively, while in silico DNA–DNA hybridization values for strain 2R12 T with these strains were 22.8 and 23.3 %, respectively. Based on comparative analysis of phylogenetic, phylogenomic, phenotypic and chemotaxonomic characteristics, strain 2R12 T represents a novel species in the genus Chitinophaga, for which the name Chitinophaga hostae sp. nov. is proposed. The type strain is 2R12 T (=ACCC 61757 T =JCM 34719 T ).