Agrilactobacillus fermenti sp. nov. isolated from fermented vegetable residue. Issue 4 (27th April 2022)

• Record Type: Journal Article
• Authors: Lin, Shih-Yao; Hameed, Asif; Tsai, Chia-Fang; Hung, Mei-Hua; Young, Chiu-Chung
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 72:Issue 4(2022)
• Publication Date: 2022-04-27
• Subjects: polyphasic taxonomic approach -- orthologous average nucleotide identity (OrthoANI) -- Agrilactobacillus fermenti; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.005336
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : A polyphasic taxonomic approach was used to characterize a Gram-stain-positive fermentative bacterium, designated strain CC-MHH1034 T, isolated from a fermented vegetable residue. Cells of strain CC-MHH1034 T were facultatively anaerobic, non-motile, and non-spore-forming rods, exhibiting positive catalase, oxidase and protease activities. Optimal growth occurred at 30 °С and pH 6.0. Strain CC-MHH1034 T shared the highest 16S rRNA gene sequence similarities with Agrilactobacillus composti (95.9 %) followed by Agrilactobacillus yilanensis (95.1 %) and established a distinct taxonomic lineage associated with these species. Highest orthologous average nucleotide identity (OrthoANI) values were recorded for strain CC-MHH1034 T versus Agrilactobacillus (71.1–71.6 %, n =2) followed by Ligilactobacillus (66.5–66.8 %, n =2), Lactobacillus (64.1–65.8 %, n =4). The mean digital DNA–DNA hybridization (dDDH) value obtained for strain CC-MHH1034 T against Agrilactobacillus was 19.2–19.5 % ( n =2). The polar lipid profile consisted of phosphatidylethanolamine, phosphatidylglycerol, two unidentified aminolipids, four unidentified glycolipids, four unidentified phospholipids and one unidentified lipid. The major polyamine was sym -homospermidine and meso -diaminopimelic acid was detected as the cell-wall peptidoglycan. The dominating cellular fatty acids (>5 %) included C16 : 0, iso -C15 : 0, anteiso -C15 : 0 and C18 : 1 ω 9c. Based on its distinct phylogenetic, phenotypic and chemotaxonomic traits together with results of comparative 16S rRNA gene sequence, OrthoANI, dDDH, and the phylogenomic placement, strain CC-MHH1034 T is considered to represent a novel species of the genus Agrilactobacillus, affiliated to the family Lactobacillaceae, for which the name Agrilactobacillus fermenti sp. nov. is proposed. The type strain is CC-MHH1034 T (=BCRC 81220 T =JCM 33476 T ).