Expanding Homogeneous Culture of Human Primordial Germ Cell–Like Cells Maintaining Germline Features Without Serum or Feeder Layers. Issue 5 (May 2022)

• Record Type: Journal Article
• Authors: Kobayashi, Mutsumi; Kobayashi, Misato; Odajima, Junko; Shioda, Keiko; Hwang, Young Sun; Sasaki, Kotaro; Chatterjee, Pranam; Kramme, Christian; Kohman, Richie E.; Church, George M.; Loehr, Amanda R.; Weiss, Robert S.; Jüppner, Harald; Gell, Joanna J.; Lau, Ching C.; Shioda, Toshi
• Is Part Of: Obstetrical & gynecological survey. Volume 77:Issue 5(2022)
• Page Start: 280
• Page End: 281
• Publication Date: 2022-05
• Subjects: Obstetrics -- Periodicals; Gynecology -- Periodicals; Generative organs, Female -- Surgery -- Periodicals; 618
• DOI: 10.1097/01.ogx.0000827980.67713.d1
• Languages: English
• ISSNs: 0029-7828

Abstract

Abstract : (Abstracted from Stem Cell Rep 2022;17(3):507–521) Primordial germ cells (PGCs) are embryonic precursors of gametes that are extensively studied in research settings. Human primordial PGC–like cells (hPGCLCs) are a surrogate model for embryonic PGCs that present difficulties in in vitro cell culture and expansion due to rapid loss of PGC-like identity and limited cell survival.