Hypermethylation of antisense long noncoding RNAs in acute lymphoblastic leukemia. (May 2017)

• Record Type: Journal Article
• Authors: Arthur, Gerald; Almamun, Md; Taylor, Kristen
• Is Part Of: Epigenomics. Volume 9:Number 5(2017)
• Page Start: 635
• Page End: 645
• Publication Date: 2017-05
• Subjects: DNA methylation -- epigenetic regulations -- lncRNA; Epigenesis -- Periodicals; Genomics -- Periodicals; Chromatin -- Periodicals; DNA -- Periodicals; 572.87
• DOI: 10.2217/epi-2016-0156
• Languages: English
• ISSNs: 1750-1911

Abstract

Aim: Long noncoding RNAs serve critical regulatory functions highly specific for a tissue and its developmental stage. Antisense long ncRNA (AS-lncRNA) methylation changes in acute lymphoblastic leukemia (ALL) versus normal pre-B-cell lymphoblasts were evaluated to identify potential differential methylation in this group of genes.Materials & methods: The methylome of ALL and normal lymphoblasts was examined by the methylated CpG island recovery assay followed by NGS.Conclusion: The potential effect of trans regulation by AS-lncRNA through DNA/RNA binding is significant as sequence alignment analysis of the 25 most differentially methylated AS-lncRNAs revealed 368 genes containing highly similar sequences with a median nucleotide identity of 90.8% and binding span of 122 base pairs. Regulation of biological processes and anatomical structure development were over represented. ALL classification schemes based on AS-lncRNA methylation can provide new insights into its pathogenesis and treatment.