BPA exposure aggravates necroptosis of myocardial tissue in selenium deficient broilers through NO-dependent endoplasmic reticulum stress. (30th April 2022)

• Record Type: Journal Article
• Authors: Liu, Jing; Chen, Ting; Wang, ShengChen; Wu, Hao; Xu, Shiwen
• Is Part Of: Toxicology. Volume 472(2022)
• Publication Date: 2022-04-30
• Subjects: Se Selenium -- BPA Bisphenol A -- ER endoplasmic reticulum -- NO nitric oxide -- NOS itric oxide synthase -- iNOS inducible nitric oxide synthase -- qRT-PCR Real time fluorescence quantitative PCR -- GRP78 glucose regulated protein 78 -- IRE1α inositol requiring protein 1α -- ATF6 activating transcription factor 6 -- CHOP C/EBP homologous protein -- PERK PRKR-like ER kinase -- elF2α eukaryotic initiation factor 2α -- XBP1 X-box binding protein 1; Bisphenol A -- Selenium deficiency -- ER stress -- Necroptosis -- Apoptosis -- Broilers; Toxicology -- Periodicals; Chemicals -- Physiological effect -- Periodicals; 615.9005
• DOI: 10.1016/j.tox.2022.153190
• Languages: English
• ISSNs: 0300-483X

Abstract

Abstract: The environmental problem of BPA pollution has seriously endangered the health of humans and animals. As an essential trace element, the health problems caused by insufficient intake of selenium have always been widespread. Under toxic and other harmful stimuli, severe endoplasmic reticulum (ER) stress is one of multitude factors leading to death such as apoptosis and necrotic apoptosis. For investigating the damage of BPA and selenium deficiency combined effect to the myocardial tissue of broilers and the role of ER stress, 1-day-old broilers were fed with toxic feed and selenium deficiency feed for 35 days. Histopathological results showed that the combined exposure of BPA and low selenium resulted in more severe necrosis of cardiomyocytes in broilers than the single exposure, but there was no significant change in apoptosis compared with single exposure. Molecular biology studies showed that NO-dependent ER stress induced by BPA increased the expression of necroptosis related genes in myocardium of selenium deficient broilers, but had no effect on apoptosis pathway. In conclusion, our records state that the NO-dependent ER stress caused by combined exposure of BPA and low selenium can cause serious damage to the myocardial tissue of broilers by promoting the activation of the necroptosis pathway. Graphical Abstract: The mechanism of BPA and selenium deficiency combined exposure aggravating necroptosis of myocardial tissue is illustrated. ga1 Highlights: BPA induces NO-dependent ER stress in broiler myocardium. Selenium deficiency induces NO-dependent ER stress in broiler myocardium. NO-dependent ER stress promotes broiler cardiomyocyte apoptosis and necroptosis. Combined exposure aggravates broiler myocardium injury mainly through necroptosis.