Influence of hematuria and infection on diagnostic accuracy of urinary LASP1: a new biomarker for bladder carcinoma. (April 2017)

• Record Type: Journal Article
• Authors: Butt, Elke; Ebbing, Jan; Bubendorf, Lukas; Ardelt, Peter
• Is Part Of: Biomarkers in medicine. Volume 11:Number 4(2017)
• Page Start: 347
• Page End: 357
• Publication Date: 2017-04
• Subjects: biomarker -- bladder cancer -- FACS -- LASP1 -- urothelial carcinoma; Biochemical markers -- Periodicals; 610.28
• DOI: 10.2217/bmm-2016-0348
• Languages: English
• ISSNs: 1752-0363

Abstract

Aim: To further promote the clinical use of urinary LASP1 as biomarker for urothelial carcinoma of the bladder regarding limitations and alternative testing systems.Patients & methods: Urine stabilization, alternative measurement systems and limitations by erythrocyte contamination and infection were investigated in 246 patients.Results: Thimerosal allowed sufficient stabilization. Fluorescence-activated cell sorting analysis was not influenced by presence of erythrocytes or leukocytes and reliably urothelial carcinoma of the bladder but cell counts in specimen were low. Cut-off values of <25 leukocytes and <200 erythrocytes/µl resulted in sensitivity, specificity, positive and negative predictive values of 0.59, 0.80, 0.80 and 0.59, respectively.Conclusion: Hematuria up to 200 erythrocytes/µl but not presence of leukocytes may be tolerated for this promising marker.