Multifunctional chitin-based hollow nerve conduit for peripheral nerve regeneration and neuroma inhibition. (1st August 2022)

• Record Type: Journal Article
• Authors: Yang, Xiaqing; Huang, Lin; Yi, Xinzeyu; Huang, Siyuan; Duan, Bo; Yu, Aixi
• Is Part Of: Carbohydrate polymers. Volume 289(2022)
• Publication Date: 2022-08-01
• Subjects: Chitin (AR, PubChem CID: 6857375) -- Sodium hydroxide (NaOH, AR, PubChem CID: 14798) -- Urea (AR, PubChem CID: 1176) -- Hydrochloric acid (HCl, AR, PubChem CID: 313) -- Epichlorohydrin (ECH, AR, PubChem CID: 7835) -- Tert-butanol (TBA, AR, PubChem CID: 6386) -- 3-Hydroxytyramine hydrochloride (dopamine hydrochloride, AR, PubChem CID: 65340) -- Tris base (Tris, AR, PubChem CID: 6503) -- Glutaraldehyde (AR, PubChem CID: 3485) -- Paraformaldehyde (AR, PubChem CID: 712) -- DAPI (AR, PubChem CID: 2954) -- Propidium iodide (PI, AR, PubChem CID: 104981); Chitin conduit -- Polydopamine -- Peripheral nerve regeneration -- Painful neuroma; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2022.119443
• Languages: English
• ISSNs: 0144-8617

Abstract

Abstract: The recoverability for peripheral nerve lesions with a long segment defect is much challenging. Conventional methods for sciatic nerve repair excepted for autografts are bridged with nerve guidance conduit (NGC). Herein, the chitin-based NGC (ChT NGC) is firstly reported by facile dissolution, molding and regeneration process, performed excellent nerve regeneration and neuroma inhibition after deposited with anti-inflammatory polydopamine (ChT-PDA NGC). In 10 mm sciatic nerve defect rat model, the restorative effects of ChT-PDA NGC groups are similar to autografts. That is mainly ascribed to the high activity of Schwann cells and claimed by immunofluorescence staining and Western blot analysis. Interestingly, ChT-PDA NGC presents outstanding neuroma inhibition during the nerve regeneration as for the anti-inflammatory activity of PDA. This work provides a facile and novel approach to prepare hollow chitin hydrogel tube, which presents well nerve regeneration and neuroma inhibition, improving the potential high-value application of chitin in biomedical fields. Graphical abstract: Chitin was dissolved in alkaline/urea solution (NaOH: urea: H2 O = 11:4:85) to acquire chitin solution through freeze-thaw method. ECH was added dropwise into chitin solution with violently stirring to ensure successful cross-linked. The cross-linked chitin solution was modeled in the as-prepared mold, and ChT NGC was obtained. The ChT NGC was immersed into Tris-HCl buffer with dopamine hydrochloride to obtain ChT-PDA NGC. Implantation of ChT-PDA NGC in sciatic nerve defect rat to assess the recovery ability. Unlabelled Image