Biochemical changes in cancer cells induced by photoactive nanosystem based on carbon dots loaded with Ru-complex. (1st June 2022)

• Record Type: Journal Article
• Authors: Nešić, Maja D.; Dučić, Tanja; Gonçalves, Mara; Stepić, Milutin; Algarra, Manuel; Soto, Juan; Gemović, Branislava; Bandosz, Teresa J.; Petković, Marijana
• Is Part Of: Chemico-biological interactions. Volume 360(2022)
• Publication Date: 2022-06-01
• Subjects: Anticancer photodynamic therapy -- Biochemical changes -- N-doped carbon dots -- Carbon dots -- Ru-metallodrug; Biochemistry -- Periodicals; Toxicological chemistry -- Periodicals; Biochemistry -- Periodicals; Biologie moléculaire -- Périodiques; Biochimie -- Périodiques; Toxicologie biochimique -- Périodiques; 572
• DOI: 10.1016/j.cbi.2022.109950
• Languages: English
• ISSNs: 0009-2797

Abstract

Abstract: Carbon dots (CDs) and N-carbon dots (N-CDs) loaded with Ru-complex (CDs@RuCN, N-CDs@RuCN, respectively) were investigated as media imposing biochemical changes induced by UV illumination of ovarian cancer, A2780, and osteosarcoma, CAL72, cells. Synchrotron radiation-based Fourier Transform Infrared Spectroscopy was performed, and the spectra were subjected to a Principal Component Analysis. The CDs@RuCN and N-CDs@RuCN effects on cancer cells were analyzed by the theoretical modelling of the stability of the composite systems and a protein database search. Moreover, a detailed evaluation of surface and optical properties of CDs@RuCN and N-CDs@RuCN was carried out. Results demonstrated selective action of the CDs@RuCN and N-CDs@RuCN-based photodynamic therapy, with N-CDs@RuCN being the most active in inducing changes in A2780 and CDs@RuCN in CAL72 cells. We assume that different surface charges of nanoparticles led to direct interactions of N-CDs@RuCN with a Wnt signalling pathway in A2780 and those of CDs@RuCN with PI3–K/Akt in CAL72 cells and that further biochemical changes occurred upon light illumination. Graphical abstract: Image 1 Highlights: Optical properties of (N-)CDs are augmented by their combination with Ru-complex. Biochemical changes depend on the surface modification of CDs and the cell type. Light-induced significant changes in A2780 cells treated with N-CDs@RuCN. CAL72 cells changes were pronounced after illumination and incubation with CDs@RuCN. Involved signalling pathways: Wnt in A2780 and Pi3-K/Akt in CAL72 cancer cells.