SdrG gene activated joint surface membrane protein PTPRJ to induce periprosthetic joint infection after total hip arthroplasty. (10th January 2022)

• Record Type: Journal Article
• Authors: Hao, Linjie; Wu, Yanjie; Zhang, Yumin; Ma, Tao; Wen, Pengfei; Zhang, Binfei; Wang, Junwei; Pei, Qi
• Is Part Of: Journal of clinical pharmacy and therapeutics. Volume 47:Number 4(2022)
• Page Start: 517
• Page End: 522
• Publication Date: 2022-01-10
• Subjects: PJI -- platelet adhesion rate -- PTPRJ -- SdrG -- THA; Clinical pharmacology -- Periodicals; Chemotherapy -- Periodicals; 615
• DOI: 10.1111/jcpt.13573
• Languages: English
• ISSNs: 0269-4727

Abstract

Abstract: What is known and objective: Up to now, no study focused on the role of SdrG in PJI after THA. To explore the mechanism of periprosthetic joint infection (PJI) after total hip arthroplasty (THA). Methods: Joint fluid and blood were collected from patients with PJI after THA, aseptic loosening of the joints or bacterial infection after traumatic fractures of the extremities alone. The expression of SdrG in the 3 groups was determined by agarose gel electrophoresis. The expression of protein tyrosine phosphatase receptor J (PTPRJ) was measured by immunohistochemistry method. The platelet adhesion rate was determined by biochemical analysis. The content of D‐dimer, CRP and ESR in blood was determined by latex agglutination method. Multiple linear correlation analysis was used to analyse the correlation between PJI and the expression of PTPRJ protein, platelet adhesion rate, D‐dimer content, CRP and ESR. Results and discussion: The expression of SdrG and PTPRJ in PJI group was markedly increased compared to the other 2 groups. The platelet adhesion rate in PJI group was markedly larger compared to aseptic loosening and simple wound infection group, and the rate in simple wound infection group was larger than aseptic loosening group. The level of D‐dimer, CRP and ESR in PJI group was higher than that of the other groups. The expression of PTPRJ protein, D‐dimer content, CRP and ESR was all closely related to PJI, while platelet adhesion rate had no correlation with PJI. What is new and conclusion: SDRG gene around joint prosthesis was over‐expressed, which activated joint surface membrane protein PTPRJ and then induced platelet aggregation to reduce joint function. Abstract : The Staphylococcus aureus or Staphylococcus epidermidis might upregulate the expression of SdrG and further increased the protein level of RTPRJ after total hip arthroplasty, resulting in the induced periprosthetic joint infection.