Metabolomic analysis of serum reveals the potential effective ingredients and pathways of Danggui Buxue Tang in promoting erythropoiesis. (January 2020)

• Record Type: Journal Article
• Authors: Wang, Xiao; Bei, Haikang; Du, Ruilan; Chen, Qizhu; Wu, Fenglin; Chen, Jun; Bo, Huaben
• Is Part Of: Complementary therapies in medicine. Volume 48(2020)
• Publication Date: 2020-01
• Subjects: DBT Danggui Buxue Tang -- BMSCs bone marrow stromal cells -- DCS drug-containing serum -- CFU-E colony forming unit-erythroid -- BFU-E burst-forming unit-erythroid -- AR Astragali Radix -- ASR Angelica Sinensis Radix; Danggui Buxue Tang -- Metabolomics -- Erythroid-related cells -- Erythrocyte; Alternative medicine -- Periodicals; Complementary Therapies -- Periodicals; Médecines parallèles -- Périodiques; Thérapeutique -- Périodiques; Alternative medicine; Electronic journals; Periodicals; 615.5
• DOI: 10.1016/j.ctim.2019.102247
• Languages: English
• ISSNs: 0965-2299

Abstract

Graphical abstract: Highlights: Metabolomic was used to reveal the mechanism of DBT in promoting erythropoiesis. 7 DBT metabolites were identified and were the potential active ingredients of DBT. The active ingredients play a therapeutic role by promoting glutamate metabolism. Abstract: Danggui Buxue Tang has been used for menopausal women in China for more than 800 years. However, the potential effective ingredients and pathways require further investigation. The main objective of this work was to explore the potential effective ingredients and pathways. The optimal administration time was optimized by detecting the changes of reticulocytes in peripheral blood. Drug-containing serum (DCS) was taken every 30 min after last administration. Because of the different concentration of effective ingredients absorbed into blood at different time, the pharmacodynamic effect is different. Therefore, bone marrow stromal cells as a member of hematopoietic microenvironment were used to evaluate the pharmacodynamics of DCS. Metabolomics was used to detect changes of metabolites (DBT and endogenous metabolites). The correlation of the metabolites and pharmacodynamics was used to identify the metabolites associated with erythropoiesis. After 14 days, the number of reticulocytes in peripheral blood, erythroid-related cells and erythroid progenitor cells in bone marrow in the DBT group were significantly increased. In vitro experiments showed that DCS at different time had different proliferation effects on BMSCs. Metabolomic analysis showed that the concentration of metabolites in DCS at different time was significantly different. The correlation analysis identified 7 DBT metabolites and 15 endogenous metabolites related to erythropoiesis. 15 endogenous metabolites were finally connected to different pathways. Glutamate is a node molecule. 7 potential effective ingredients of DBT were found. DBT promoted erythropoiesis via promoting the metabolism of glutamate and further affect other pathways.