Antibacterial action of nitric oxide-releasing hyperbranched polymers against ex vivo dental biofilms. Issue 5 (May 2020)

• Record Type: Journal Article
• Authors: Yang, Lei; Teles, Flavia; Gong, Weida; Dua, Shawn A.; Martin, Lynn; Schoenfisch, Mark H.
• Is Part Of: Dental materials. Volume 36:Issue 5(2020)
• Page Start: 635
• Page End: 644
• Publication Date: 2020-05
• Subjects: Periodontal disease -- Antibacterial agent -- Nitric oxide -- Hyperbranched polymer -- Multispecies biofilms -- Ex vivo; Dentistry -- Periodicals; Dental materials -- Periodicals; 617.695
• DOI: 10.1016/j.dental.2020.03.012
• Languages: English
• ISSNs: 0109-5641

Abstract

Graphical abstract: Abstract: Objectives: This study investigates the antibiofilm action of nitric oxide (NO)-releasing hyperbranched polymers against ex vivo multispecies periodontal biofilms. Methods: The antibiofilm efficacy of NO-releasing hyperbranched polymers was evaluated as a function of NO-release properties, polymer concentrations, and oxygen levels in the exposure media. 16s rRNA sequencing technique was employed to evaluate the impact of NO-releasing hyperbranched polymers on the microbial composition of the biofilms. Results: The addition of NO release significantly improved the antibiofilm action of the hyperbranched polymers, with NO-releasing hyperbranched polyamidoamines of largest NO payloads being more effective than hyperbranched polykanamycins. Furthermore, the NO-releasing hyperbranched polymers reduced the biofilm metabolic activity in a dose-dependent manner, killing biofilm-detached bacteria under both aerobic and anaerobic conditions, with greater antimicrobial efficacy observed under aerobic conditions. Significance: These results demonstrate for the first time the potential therapeutic utility of NO-releasing hyperbranched polymers for treating multispecies dental biofilms.