SCCmecIX in meticillin‐resistantStaphylococcus aureusand meticillin‐resistant coagulase‐negative staphylococci from pigs and workers at pig farms in Khon Kaen, Thailand. Issue 9 (September 2015)

• Record Type: Journal Article
• Authors: Sinlapasorn, Siwaporn; Lulitanond, Aroonlug; Angkititrakul, Sunpetch; Chanawong, Aroonwadee; Wilailuckana, Chotechana; Tavichakorntrakool, Ratree; Chindawong, Kanoksak; Seelaget, Charinya; Krasaesom, Mana; Chartchai, Sarawut; Wonglakorn, Lumyai; Sribenjalux, Pipat
• Is Part Of: Journal of medical microbiology. Volume 64:Issue 9(2015)
• Publication Date: 2015-09
• Subjects: Medical microbiology -- Periodicals; 616.9041
• DOI: 10.1099/jmm.0.000119
• Languages: English
• ISSNs: 0022-2615

Abstract

Abstract : N/A: Livestock‐associated meticillin‐resistant Staphylococcus aureus, clonal complex (CC) 398, has been reported in Europe, whereas CC9 MRSA has mostly been found in Asia. Therefore, we aimed to detect MRSA on pig farms in north‐eastern Thailand. A total of 257 nasal swabs (159 samples from pigs and 98 from pig‐farm workers) were collected from three pig farms in north‐eastern Thailand from 2010 to 2011. MRSA isolates were confirmed for femA and mecA genes by PCR. The MICs of eight antimicrobials, namely vancomycin (VA), cefazolin (CZ), ofloxacin (OF), tetracycline (TET), erythromycin (ER), oxacillin (OX), cefoxitin (FOX) and gentamicin (GN), were tested by agar dilution method. The virulence genes for Panton–Valentine leukocidin toxin ( lukSF‐PV ), toxic shock syndrome toxin‐1 ( tst ) and α‐haemolysin ( hla ) were detected by PCR. Strain typing was performed by staphylococcal cassette chromosome (SCC) mec, agr, spa and multilocus sequence typing. Four MRSA were isolated: three from workers and one from a pig. All the MRSA isolates were resistant to OX, GN, ER, TET and CZ, and they all carried hla only. Two MRSA from humans carried SCC mec II‐sequence type (ST)764‐ agr II, whereas the two remaining MRSA (one each from a human and a pig) contained SCC mec IX‐ST9‐ agr II. Interestingly, meticillin‐resistant coagulase‐negative Staphylococcus isolates carrying SCC mec IX were also obtained from five workers and three pigs. This study suggests that the SCC mec IX element is distributed among the Staphylococcus found in pigs and pig‐farm workers, and pigs may be a reservoir for MRSA in the community.