Current methods to analyze lysosome morphology, positioning, motility and function. (24th April 2022)

• Record Type: Journal Article
• Authors: Barral, Duarte C.; Staiano, Leopoldo; Guimas Almeida, Cláudia; Cutler, Dan F.; Eden, Emily R.; Futter, Clare E.; Galione, Antony; Marques, André R. A.; Medina, Diego Luis; Napolitano, Gennaro; Settembre, Carmine; Vieira, Otília V.; Aerts, Johannes M. F. G.; Atakpa‐Adaji, Peace; Bruno, Gemma; Capuozzo, Antonella; De Leonibus, Elvira; Di Malta, Chiara; Escrevente, Cristina; Esposito, Alessandra; Grumati, Paolo; Hall, Michael J.; Teodoro, Rita O.; Lopes, Susana S.; Luzio, J. Paul; Monfregola, Jlenia; Montefusco, Sandro; Platt, Frances M.; Polishchuck, Roman; De Risi, Maria; Sambri, Irene; Soldati, Chiara; Seabra, Miguel C.;
• Is Part Of: Traffic. Volume 23:Number 5(2022)
• Page Start: 238
• Page End: 269
• Publication Date: 2022-04-24
• Subjects: endolysosomes -- lysosomal storage diseases -- lysosome biogenesis -- lysosome exocytosis -- lysosome‐related organelles -- lysosomes -- membrane contact sites -- mTOR -- TFEB; Biological transport -- Periodicals; 571.6
• DOI: 10.1111/tra.12839
• Languages: English
• ISSNs: 1398-9219

Abstract

Abstract: Since the discovery of lysosomes more than 70 years ago, much has been learned about the functions of these organelles. Lysosomes were regarded as exclusively degradative organelles, but more recent research has shown that they play essential roles in several other cellular functions, such as nutrient sensing, intracellular signalling and metabolism. Methodological advances played a key part in generating our current knowledge about the biology of this multifaceted organelle. In this review, we cover current methods used to analyze lysosome morphology, positioning, motility and function. We highlight the principles behind these methods, the methodological strategies and their advantages and limitations. To extract accurate information and avoid misinterpretations, we discuss the best strategies to identify lysosomes and assess their characteristics and functions. With this review, we aim to stimulate an increase in the quantity and quality of research on lysosomes and further ground‐breaking discoveries on an organelle that continues to surprise and excite cell biologists. Abstract : Lysosomes play essential roles beyond their well‐known degradative function, including nutrient sensing, intracellular signalling and metabolism. To better understand these functions, it is essential to employ the best methodological approaches and know their advantages and limitations. Here, we review current methods used to analyze lysosome morphology, positioning, motility and function. The proper use of these methods will allow new discoveries to be made about this fascinating organelle.