Carboxymethylation modification, characterization, antioxidant activity and anti-UVC ability of Sargassum fusiforme polysaccharide. (May 2022)

• Record Type: Journal Article
• Authors: An, Yongzhen; Liu, Haitang; Li, Xuexiu; Liu, Jing; Chen, Lin; Jin, Xin; Chen, Ting; Wang, Wenqian; Liu, Zhong; Zhang, Meiyun; Liu, Fufeng
• Is Part Of: Carbohydrate research. Volume 515(2022)
• Publication Date: 2022-05
• Subjects: Sargassum fusiforme Polysaccharide -- Carboxymethylation -- Characterization -- Biological activity; Carbohydrates -- Periodicals; Chemistry, Organic -- Periodicals; Biochemistry -- Periodicals; Carbohydrates -- Periodicals; Chimie organique -- Périodiques; Glucides -- Périodiques; Biochemistry; Carbohydrates; Chemistry, Organic; Periodicals; Electronic journals; 507.78
• DOI: 10.1016/j.carres.2022.108555
• Languages: English
• ISSNs: 0008-6215

Abstract

Abstract: Taking the degree of substitution (DS) as the index, the carboxymethylation conditions of Sargassum fusiforme polysaccharide (SFP) were studied. According to the single factor experiment results, the optimum experimental conditions were obtained: sodium hydroxide concentration, 15% (20 mL); alkalization temperature, 50 °C; dosage of chloroacetic acid 1.5 g; etherification time, 2 h, and the Carboxymethyl Sargassum fusiforme polysaccharide (CSFP) with the highest DS (0.635) was obtained. And then, the physicochemical properties, structural information and bioactivity of SFP and CSFP were characterized. The SFP and CSFP were composed of four monosaccharides, with a small amount of protein, and their molecular weights to 780.2 kDa and 386.3 kDa respectively. The results of FTIR and NMR showed that the carboxymethyl was successfully grafted onto the C-4 and C-6 of sugar chain. The results of anti UVC experiment showed that SFP and CSFP had a certain negative effect on cell activity, and the degree of damage caused by UVC radiation was weakened, and the anti UVC performance of CSFP was better than that of SFP. Graphical abstract: Image 1 Highlights: The carboxymethylation of SFP was proposed. The positions of carboxymethyl substitution were on the C-4 and C-6 of the glucose. The CSFP has the better Oxidation resistance and anti-UVC ability. SFP and CSFP have broad application prospects in the field of cosmetics.