Cytomegalovirus infections in infants in Uganda: Newborn-mother pairs, neonates with sepsis, and infants with hydrocephalus. (May 2022)

• Record Type: Journal Article
• Authors: Hehnly, Christine; Ssentongo, Paddy; Bebell, Lisa M.; Burgoine, Kathy; Bazira, Joel; Fronterre, Claudio; Kumbakumba, Elias; Mulondo, Ronald; Mbabazi-Kabachelor, Edith; Morton, Sarah U.; Ngonzi, Joseph; Ochora, Moses; Olupot-Olupot, Peter; Mugamba, John; Onen, Justin; Roberts, Drucilla J.; Sheldon, Kathryn; Sinnar, Shamim A.; Smith, Jasmine; Ssenyonga, Peter; Kiwanuka, Julius; Paulson, Joseph N.; Meier, Frederick A.; Ericson, Jessica E.; Broach, James R.; Schiff, Steven J.
• Is Part Of: International journal of infectious diseases. Volume 118(2022)
• Page Start: 24
• Page End: 33
• Publication Date: 2022-05
• Subjects: cytomegalovirus infection -- sub-Saharan Africa -- hydrocephalus -- neonatal sepsis; Communicable diseases -- Periodicals; Communicable Diseases -- Periodicals; Communicable diseases; Periodicals; Electronic journals; 616.9
• DOI: 10.1016/j.ijid.2022.02.005
• Languages: English
• ISSNs: 1201-9712

Abstract

Abstract: Objectives: To estimate the prevalence of cytomegalovirus (CMV) infections among newborn-mother pairs, neonates with sepsis, and infants with hydrocephalus in Uganda. Design and Methods: Three populations—newborn-mother pairs, neonates with sepsis, and infants (≤3 months) with nonpostinfectious (NPIH) or postinfectious (PIH) hydrocephalus—were evaluated for CMV infection at 3 medical centers in Uganda. Quantitative PCR (qPCR) was used to characterize the prevalence of CMV. Results: The overall CMV prevalence in 2498 samples across all groups was 9%. In newborn-mother pairs, there was a 3% prevalence of cord blood CMV positivity and 33% prevalence of maternal vaginal shedding. In neonates with clinical sepsis, there was a 2% CMV prevalence. Maternal HIV seropositivity (adjusted odds ratio [aOR] 25.20; 95% confidence interval [CI] 4.43–134.26; p = 0.0001), residence in eastern Uganda (aOR 11.06; 95% CI 2.30–76.18; p = 0.003), maternal age <25 years (aOR 4.54; 95% CI 1.40–19.29; p = 0.02), and increasing neonatal age (aOR 1.08 for each day older; 95% CI 1.00–1.16; p = 0.05), were associated risk factors for CMV in neonates with clinical sepsis. We found a 2-fold higher maternal vaginal shedding in eastern (45%) vs western (22%) Uganda during parturition (n = 22/49 vs 11/50, the Fisher exact test; p = 0.02). In infants with PIH, the prevalence in blood was 24% and in infants with NPIH, it was 20%. CMV was present in the cerebrospinal fluid (CSF) of 13% of infants with PIH compared with 0.5% of infants with NPIH (n = 26/205 vs 1/194, p < 0.0001). Conclusions: Our findings highlight that congenital and postnatal CMV prevalence is substantial in this African setting, and the long-term consequences are uncharacterized. Graphical abstract: Image, graphical abstract