A fluorescent probe derived from benzofuranone for turn-on detection of sulfite in living cells and mice. (June 2022)

• Record Type: Journal Article
• Authors: Guo, Meng-Ya; Wei, Lu-Gang; Shen, Jia-Wen; Liu, Xiao-Jing; Abbas, Muhammad; Yang, Yu-Shun; Xu, Chen; Wang, Bao-Zhong; Zhu, Hai-Liang
• Is Part Of: Dyes and pigments. Volume 202(2022)
• Publication Date: 2022-06
• Subjects: Fluorescent probe -- Benzofuranone derivative -- Detection of sulfite -- Biological imaging; Dyes and dyeing -- Periodicals; Pigments -- Periodicals; 667.2
• DOI: 10.1016/j.dyepig.2022.110261
• Languages: English
• ISSNs: 0143-7208

Abstract

Abstract: A novel benzofuranone fluorescent probe, BFO-SFT, for monitoring sulfite in living cells and mice is described which operates over a wide pH range and exhibits a red-shifted emission maximum. The response of the probe BFO-SFT was relatively rapid (within 20 min) and exhibited a good linear correlation of 0–10 Eq (100 μM) with reliable accuracy (R 2 = 0.9934). The probe also showed high sensitivity (Limit of detection = 152 nM) and selectivity. With low cytotoxicity, BFO-SFT was successfully applied into the biological imaging of sulfite in living MCF-7 cells and in BALB/C Nude mice. Graphical abstract: Image 1 Highlights: A benzofuranone-based fluorescent probe, BFO-SFT, was developed for monitoring sulfite. The benzofuranone backbone achieved the purposes of introducing red-shift and wide pH window (7.0–10.0). Reliable linear range, high sensitivity and high selectivity towards sulfite. Applied into the biological imaging of sulfite in living MCF-7 cells and in BALB/C Nude mice.