AB0042 Essential oil extracted from chamaecyparis obtusa attenuates the severity of collagen-induced arthritis. (12th June 2018)

• Record Type: Journal Article
• Authors: Lee, J.; Park, Y.; Yoon, C.-H.
• Is Part Of: Annals of the rheumatic diseases. Volume 77(2018)Supplement 2
• Page Start: 1221
• Page End: 1221
• Publication Date: 2018-06-12
• Subjects: Rheumatism -- Periodicals; 616.723005
• DOI: 10.1136/annrheumdis-2018-eular.1413
• Languages: English
• ISSNs: 0003-4967

Abstract

Abstract : Background: Chamaecyparis obtusa ( C. obtusa ) is a tropical tree species found in Japan and the southern region of South Korea. Although the biological activities of essential oil extracted from C. obtusa (EOCO) are not yet fully understood, essential oil has been shown to have antibacterial activities and anti-inflammatory effects. Objectives: We investigated the effect of essential oil extracted from Chamaecyparis obtusa on the development of collagen-induced arthritis (CIA), a well-characterised preclinical model of human RA. Methods: DBA1/J mice were injected intraperitoneally with EOCO dissolved in dimethyl sulfoxide (DMSO) 3 times a week for 5 weeks after type II collagen (CII) immunisation. Arthritis severity was evaluated by clinical and histological parameters. Serum anti-CII antibody levels were assayed by ELISA. Iinterleukin-17 (IL-17) expressions in the joints were determined by immunohistochemistry. Effect of EOCO on Th17 cells and natural killer (NK) cells was analysed using flow cytometry. Results: EOCO markedly reduced the clinical and histological severity of CIA. Serum anti-CII antibody levels and the expression of IL-17 in the joints were significantly lower in mice treated with EOCO than those treated with DMSO alone. EOCO inhibited the differentiation of Th17 cells in total splenocytes, not in isolated CD4 + T cells, from mice. EOCO increased the production of granzyme B and perforin in NK cells. Conclusions: Our data demonstrate a protective effect of EOCO on the development of CIA through the inhibition of Th17 cell differentiation. This effect may be related with NK cell activity enhanced by EOCO. References: [1] Hong EJ, Na KJ, Choi IG, Choi KC, Jeung EB. Antibacterial and antifungal effects of essential oils from coniferous trees. Biol Pharm Bull2004;27:863–6. [2] An BS, Kang JH, Yang H, Jung EM, Kang HS, Choi IG, et al. Anti-inflammatory effects of essentia oils from Chamaecyparis obtusa via the cyclooxygenase-2 pathway in rats. Mol Med Rep2013;8:255–9. Acknowledgements: This study was carried out with the support of 'Forest Science and Technology Projects (Project No. S111114L020100)' provided by Korea Forest Service. Disclosure of Interest: None declared