A Sandwich‐type Electrochemical Immunosensor for the Sensitive Determination of Salmonella Typhimurium in Food. Issue 5 (13th January 2022)

• Record Type: Journal Article
• Authors: Sun, Qiaoling; Liu, Xiaojun; Tang, Haowen; Qian, Yuying; Gu, Haiying; He, Hong
• Is Part Of: Electroanalysis. Volume 34:Issue 5(2022)
• Page Start: 911
• Page End: 918
• Publication Date: 2022-01-13
• Subjects: Electrochemical Immunosensor -- Food Control -- Sandwich-type -- Salmonella Typhimurium; Electrochemical analysis -- Periodicals; Chemistry, Analytical -- Periodicals; Electrochemistry -- Periodicals; 543.4
• DOI: 10.1002/elan.202100561
• Languages: English
• ISSNs: 1040-0397

Abstract

Abstract: A sandwich‐type electrochemical immunosensor was developed for sensitive detection of Salmonella Typhimurium ( S . Typhimurium) in milk and eggs. According to the antigen‐antibody specific binding, Anti‐ S . Typhimurium antibody was used to recognize the S . Typhimurium, while the HRP‐Anti‐ S . Typhimurium antibody as signal probe was used to catalyze H2 O2 with the assistance of thionine. The immunosensor showed wide linear range from 5.6×10 1 to 5.6×10 8 CFU/mL and low detection limit of 35 CFU/mL. The immunosensor also exhibited excellent specificity, reproducibility and stability, indicating that it had practicability in quality control of complex food samples and potential in clinical applications. Abstract :