AB0139 Investigation of prevotella copri from rheumatoid arthritis patients. (12th June 2018)

• Record Type: Journal Article
• Authors: Maeda, Y.; Motooka, D.; Nii, T.; Matsumoto, Y.; Matsushita, M.; Saeki, Y.; Narazaki, M.; Kumanogoh, A.; Nakamura, S.; Takeda, K.
• Is Part Of: Annals of the rheumatic diseases. Volume 77(2018)Supplement 2
• Page Start: 1261
• Page End: 1261
• Publication Date: 2018-06-12
• Subjects: Rheumatism -- Periodicals; 616.723005
• DOI: 10.1136/annrheumdis-2018-eular.5350
• Languages: English
• ISSNs: 0003-4967

Abstract

Abstract : Background: We have previously reported some of the rheumatoid arthritis (RA) patients had Prevotella copri in the intestine. By using germ-free (GF) SKG mice, we also showed that Prevotella –dominated gut microbiota contribute to the development of arthritis 1) . However, P. copri itself has not been isolated from RA patients and their molecular biology was unknown. Objectives: Firstly, we planned to evaluate the intestinal microbiota in RA patients before and after the treatment. Second, we isolated P. copri strains from RA patients and healthy controls (HCs) and analysed whether RA patients-derived P. copri expanded in the intestine of GF mice. Methods: We first examined whether RA patients have altered composition of microbiota. All the patients were diagnosed according to the American College of Rheumatology/ European League Against Rheumatism 2010 classification criteria for RA. We collected faecal samples from 55 RA patients (61.5±9.5 years, mean ages±SD) and 33 HCs (56.2±8.2 years) to investigate the microbiota by 16S rRNA-based deep sequence technique. We also analysed bacterial counts of Prevotella and Bacteroides fragilis by qPCR method. Moreover, we isolated P. copri from faecal contents of RA patients and HCs. GF mice were inoculated with P. copri from RA patients and HCs for further analysis. Results: We found that 34.5% (19/55) of RA patients and 18.1% (6/33) of healthy controls have relatively high abundance of Prevotella (>4%) in the intestine. These results were compatible with our previous observations. When we focused on the patients who harboured high abundance of Prevotella in the gut, Prevotella/B. fragilis ratio was decreased after the treatment. The mean Prevotella/B. fragilis ratio were changed from 1.28 to 0.75 (p=0.08). Further analyses revealed that RA patients-derived P. copri successfully colonised to GF-mice and induced Th17 cells in the large intestine. Conclusions: We found that alternation of microbiota composition was observed after the RA treatment. Moreover, we successfully isolated P. copri from RA patients and HCs. RA patients-derived P. copri efficiently expanded in the intestine of GF mice. Reference: [1] Maeda Y, et al. Dysbiosis contributes to arthritis development via activation of autoreactive T cells in the intestine. Arthritis Rheumatol2016;68(11):2646–2661. Disclosure of Interest: None declared