Can calf age be estimated using a combination of serum gamma-glutamyl transferase, total protein and immunoglobulin G?. (December 2021)

• Record Type: Journal Article
• Authors: Roadknight, Natalie; Wales, William; Jongman, Ellen; Courtman, Natalie; Mansell, Peter; Woodward, Andrew P.; Fisher, Andrew
• Is Part Of: Research in veterinary science. Volume 141(2021)
• Page Start: 14
• Page End: 18
• Publication Date: 2021-12
• Subjects: Blood -- Biochemistry -- Non-replacement calf -- Dairy calf -- Colostrum -- Antibody; Veterinary medicine -- Periodicals; Veterinary Medicine -- Periodicals; Médecine vétérinaire -- Périodiques; Médecine vétérinaire -- Recherche -- Périodiques; Diergeneeskunde; 636.089
• DOI: 10.1016/j.rvsc.2021.10.002
• Languages: English
• ISSNs: 0034-5288

Abstract

Abstract: Our aim was to test a novel method for estimating the age of young calves, using serum γ-glutamyl transferase (GGT) activity, total protein concentration and immunoglobulin G (IgG) concentration. Blood samples were taken from 59 dairy calves at 2, 4, 6, 8, 10, 12, and 14 days of age, and serum GGT activity, total protein concentration, and IgG concentration were measured. Bayesian regression models were used to estimate the association of GGT, total protein, and IgG, with calf age. A multivariate hierarchical regression model was then created, and the model's reliability in estimating the age of simulated subjects was assessed. The univariate models showed a strong within-calf relationship between age and GGT (conditional R 2 (cR 2 ) = 0.93), and age and total protein (cR 2 = 0.75), while the relationship between calf age and IgG was less consistent (cR 2 = 0.63). There was a high degree of variation between calves for these parameters, particularly for the relationship between age and total protein (marginal R 2 (mR 2 ) 0.02), and age and IgG (mR 2 0.01); somewhat less between-calf variation was seen for GGT (mR 2 0.30). For the final multivariate model, we tested the model reliability by simulating new subjects. The credible intervals for estimates of calf age generated from the model were wide, indicating poor reliability. We concluded that single measurements of serum GGT activity, total protein concentrations and IgG concentrations cannot be used to reliably estimate the age of young calves to within 1–2 days. Highlights: Serum γ-glutamyl transferase (GGT) activity decreases with age in young calves. Serum total protein and immunoglobulin G (IgG) are relatively stable in early life. We modelled calf age as a function of GGT, total protein and IgG. Model age estimates were not reliable, due to variability between individuals. Serum GGT, total protein and IgG cannot be used to reliably estimate calf age.